We narrowed to 15,812 results for: 110

1. Flag Depdc5 pLJM1

   Plasmid

   #46336

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Differs from Depdc5 isoform 1 by 10 amino acids …

   Promoter

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pLenti-CaMKIIa-SpyTag-C1C2-mCherry

   Plasmid

   #69833

   Purpose

   Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing SpyTag-C1C2-mCherry fusion. SpyTag can be used to monitor membrane localization of the C1C2 opsin.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

   Insert

   SpyTag-C1C2
   

   Use

   Lentiviral

   Tags

   Trafficking signal and mCherry

   Expression

   Mammalian

   Mutation

   Inserted SpyTag after the signal peptide of C1C2.

   Promoter

   CamKIIa

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIBN-tagBFP-TetR

   Plasmid

   #103797

   Purpose

   BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-tagBFP-TetR (CIB1, tetR E. coli, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TetR: A4G (M2V)

   Promoter

   CMV

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N1 VAMP2 R125TAG

   Plasmid

   #69876

   Purpose

   express in mammalian cells for unnatural amino acid incorporation into VAMP2-GFP

   Depositor

   Silvio Rizzoli

   Article

   Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

   Insert

   VAMP2-GFP (Vamp2 Rat)
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Amber stop codon in the linker between VAMP2 and …

   Promoter

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

5. mKate1.3-Fibrillarin-7

   Plasmid

   #56084

   Purpose

   Localization: Nucleoli, Excitation: 588, Emission: 635

   Depositor

   Michael Davidson

   Article

   Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

   Insert

   Fibrillarin (FBL Human)
   

   Use

   Tags

   mKate1.3

   Expression

   Mammalian

   Mutation

   R36Q, S66F

   Promoter

   CMV

   Available since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pMXs-ms-c-Myc-V394D

   Plasmid

   #50775

   Purpose

   retroviral expression of mouse c-Myc V394D mutant

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   c-Myc V394D (Myc Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   V394D (does not bind to Miz-1)

   Promoter

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-H1-shRNA-CMV-HA-PACSIN1

   Plasmid

   #72577

   Purpose

   Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue construct

   Depositor

   Richard Huganir

   Article

   Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

   Inserts

   PACSIN1 (Pacsin1 Mouse)
   H1-PACSIN1 shRNA cassette
   

   Use

   RNAi

   Tags

   HA

   Expression

   Mammalian

   Mutation

   5 silent mutations around shRNA recognition seque…

   Promoter

   CMV and H1

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Human_TFAM_NoMTS_L6_pET28a+

   Plasmid

   #60012

   Purpose

   Expresses TFAM No MTS L6 mutant in bacterial cells

   Depositor

   David Chan

   Article

   Ngo et al Nat Commun. 2014;5:3077. doi: 10.1038/nc

   Insert

   Human TFAM No MTS (TFAM Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Missing 1st 42 aas (Mitochondrial targeting seque…

   Promoter

   T7

   Available since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. psiCHECK2-E2F3 3'UTR

   Plasmid

   #29470

   Depositor

   Judy Lieberman

   Article

   Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

   Insert

   E2F3 3'UTR (E2F3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pTER GAMMA-TUBULIN shRNA Human

    Plasmid

    #87955

    Purpose

    reduced the expression of gamma-tubulin in human cells

    Depositor

    Ana Carrera, Maria Alvarado-Kristensson

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    sh gamma-tubulin (TUBG1 Human)
    

    Use

    Tags

    No-tag

    Expression

    Mammalian

    Mutation

    The protein is a sh-gamma-tubulin resistant gene.…

    Promoter

    Available since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-neo-myc-LC3(deltaC22, G120A)

    Plasmid

    #45449

    Depositor

    Tamotsu Yoshimori

    Article

    Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Encodes amino acids 1-120, Glycine at position 12…

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pMXs-hu-c-Myc-dN2

    Plasmid

    #50774

    Purpose

    retroviral expression of human c-Myc dN2 fragment

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    c-Myc -dN2 (MYC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    N-terminal deletion, contains aa 42-439

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs-hu-c-Myc-dN1

    Plasmid

    #50773

    Purpose

    retroviral expression of human c-Myc dN1 fragment

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    c-Myc -dN1 (MYC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    N-terminal deletion, contains aa 14-439

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCIBN-EYFP-hPMLIII

    Plasmid

    #103808

    Purpose

    BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hPMLIII (CIB1, PML Mustard Weed, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 1068 pGL3 FasL promoter (no SV40 prom)

    Plasmid

    #9029

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    Fas ligand promoter (FASLG Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Removed the SV40 promoter from pGL3

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pDEST27-GST-ATXN1 FL(92Q)-S776D

    Plasmid

    #21757

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 92 Q(Glutamines) and…

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

17. integrin beta6 777T pcDNA1 neo

    Plasmid

    #13582

    Depositor

    Dean Sheppard

    Article

    Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

    Insert

    integrin beta 6 777T (ITGB6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    777T. Lacks last 11 amino acids of cytoplasmic do…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pN1 VAMP2 R125TAG

    Plasmid

    #69877

    Purpose

    express in mammalian cells for unnatural amino acid incorporation into VAMP2

    Depositor

    Silvio Rizzoli

    Article

    Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Amber and Ochre stop codons in the former linker …

    Promoter

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pDEST27-GST-ATXN1 FL(30Q)-S776D

    Plasmid

    #21755

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 32 amino acids (30 Q…

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

20. PIK3CD gRNA (BRDN0001146545)

    Plasmid

    #76165

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3CD

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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