We narrowed to 7,217 results for: ARC;

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pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)

Plasmid

#226990

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA 1b to create OPA1-del_exon5

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A9-gRNA_(CJT87)

Plasmid

#226988

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A9)

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA A9 to create OPA1-R194G

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-ACG-IRES-mCherry

Plasmid

#222113

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Mutation

ACG start codon

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-UUG-IRES-mCherry

Plasmid

#222112

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Mutation

UUG start codon

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-AUU-IRES-mCherry

Plasmid

#222118

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Mutation

AUU start codon

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-AGG-IRES-mCherry

Plasmid

#222115

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Mutation

AGG start codon

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-AUC-IRES-mCherry

Plasmid

#222117

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Mutation

AUC start codon

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-REEP1 scFv [N345/51 scFv]

Plasmid

#219493

Purpose

Mammalian Expression Plasmid of anti-REEP1 (Mouse) scFv. Derived from hybridoma N345/51.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-REEP1 (Mus musculus) recombinant mouse scFv. (Reep1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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Calretinin scFv [L122/68]

Plasmid

#182077

Purpose

Mammalian Expression of Calretinin scFv. Derived from hybridoma L122/68.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Calretinin (Homo sapiens) (CALB2 Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-SFFV_NKX2-1_Short_V5-GFP

Plasmid

#221500

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an SFFV promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Promoter

SFFV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_HD_160-223AA

Plasmid

#221477

Purpose

Gateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway Cloning

Mutation

Deletion of 1-159AA N-terminal and 224-371AA C-te…

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-hPGK_NKX2-1_Short_V5-GFP

Plasmid

#221498

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an hPGK promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Promoter

hPGK

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_BCL11A:1-773

Plasmid

#211015

Purpose

Mammalian expression of full-length human BCL11A. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

BCL11A:M1-F773 (BCL11A Human)

Tags

HaloTag

Expression

Mammalian

Mutation

isoform 2

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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TRIP8b (exon 4) scFv [N212/3]

Plasmid

#211607

Purpose

Mammalian Expression Plasmid of TRIP8b (exon 4) scFv. Derived from hybridoma N212/3.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

TRIP8b (exon 4) (Rattus norvegicus) recombinant mouse scFv. (Pex5l Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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SynDIG1/Tmem90b scFv [L42/17]

Plasmid

#211602

Purpose

Mammalian Expression Plasmid of SynDIG1/Tmem90b scFv. Derived from hybridoma L42/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

SynDIG1/Tmem90b (Rattus norvegicus) recombinant mouse scFv. (Syndig1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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NALCN ion channel scFv [N185/7]

Plasmid

#206721

Purpose

Mammalian Expression of NALCN ion channel scFV. Derived from hybridoma N185/7 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

NALCN ion channel (Rattus norvegicus) recombinant scFV (Nalcn Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Shank1 and Shank3 scFv [N367/51]

Plasmid

#206728

Purpose

Mammalian Expression of Shank1 and Shank3 scaffold protein scFV. Derived from hybridoma N367/51 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Shank1 and Shank3 scaffold protein (Rattus norvegicus) recombinant scFV (Shank3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Kvbeta2 K+ channel scFv [K17/70]

Plasmid

#206729

Purpose

Mammalian Expression of Kvbeta2 K+ channel scFV. Derived from hybridoma K17/70 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kvbeta2 K+ channel (Mus musculus) recombinant scFV (Kcnab2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Olig1 scFv [N149/25]

Plasmid

#206732

Purpose

Mammalian Expression of Olig1 scFV. Derived from hybridoma N149/25 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Olig1 (Mus musculus) recombinant scFV (Olig1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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SALM2/LRFN1 scFv [N52B/27]

Plasmid

#206747

Purpose

Mammalian Expression of SALM2/LRFN1 scFV. Derived from hybridoma N52B/27 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

SALM2/LRFN1 (Rattus norvegicus) recombinant scFV (Lrfn1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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