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pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231363

Purpose

Ple155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231367

Purpose

Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

Plasmid

#231368

Purpose

Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

mRuby2

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-Ple155-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231351

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from Ple155 element, active in Purkinje cells.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-CMVe-SCP1-TagBFP2-W3SL-BC(p1-10)

Plasmid

#231354

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter and CMV enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

Plasmid

#231345

Purpose

Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7/T3-BC(p1-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-mproSAAS (27 kDa)

Plasmid

#231635

Purpose

Mammalian expression of a flag-tagged full-length version of the mouse chaperone protein proSAAS.

Depositor

Iris Lindberg

Article

Peinado et al ACS Chem Neurosci. 2022 Jun 1;13(11):165

Insert

Pcsk1N (Pcsk1n Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_FLAG_hSF3B1

Plasmid

#176590

Purpose

Adenoviral construct to insert a N-terminal FLAG to one allele of human SF3B1

Depositor

Manoj Pillai

Article

Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

Insert

SF3B1 (SF3B1 Human)

Use

AAV

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSuper DGK alfa human

Plasmid

#224574

Purpose

To downregulate the expression of human DGK alfa in human cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Immunol Cell Biol. 2017 Jul;95(6):549-56

Insert

sh RNAi Diacylglycerol kinase alfa human (DGKA Human)

Use

RNAi

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSuper DGK alfa mouse

Plasmid

#224573

Purpose

Negative Control for expression downregulation of DGK alfa human in human cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Immunol Cell Biol. 2017 Jul;95(6):549-56

Insert

sh RNAi Diacylglycerol kinase alfa mouse (Dgka Mouse)

Use

RNAi

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-838

Plasmid

#217994

Purpose

pLEXSY_I-blecherry3_dom_lacZ Destination Vector

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Type

Empty backbone

Use

Synthetic Biology

Promoter

T7 promotor

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-mVprBP pc2953

Plasmid

#201698

Purpose

Expresses of GFP-tagged VprBP in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

VprBP (Dcaf1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pmcherry-mVprBP pc2954

Plasmid

#201699

Purpose

Expresses of mcherry-tagged mVprBP in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

VprBP (Dcaf1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-TEX264wt-cFlag

Plasmid

#220204

Purpose

Mammalian expression of wild type TEX264, C-terminal Flag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-TEX264-E194A-cFlag

Plasmid

#220207

Purpose

Mammalian expression of TEX264 with E194A mutation, C-terminal Flag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

Flag

Expression

Mammalian

Mutation

194E>A

Promoter

CMV

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-ccdB-SNAP

Plasmid

#166146

Purpose

Gateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

His10 and SNAP

Expression

Bacterial

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-Smt3-ccdB-SNAP

Plasmid

#166148

Purpose

Gateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

His10-Smt3 and SNAP

Expression

Bacterial

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

Plasmid

#189633

Purpose

Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

Plasmid

#189632

Purpose

Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pNK005C_BattB_mkozak_STIM1_IRES_mCherry-H2A-P2A-PuroR

Plasmid

#200639

Purpose

Low STIM1 abundance recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

STIM1 (STIM1 Human)

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 PSK (745-1235)

Plasmid

#197863

Purpose

Expression of C-terminal PSK1 / TAOK2 (aa745-1235) in mammalian cells (microtubule-binding domain)

Depositor

Jonathan Morris

Article

Mitsopoulos et al J Biol Chem. 2003 May 16;278(20):18085-9

Insert

PSK1 (745-1235) (TAOK2 Human)

Tags

Myc

Expression

Mammalian

Mutation

The N terminus of PSK1/TAOK2 has been deleted

Promoter

SV40

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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CB6-GFP-TAOK1 (K57A)

Plasmid

#197109

Purpose

Expression of kinase-deficient GFP-tagged TAOK1 (K57A) / PSK2 (K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

Insert

TAOK1 (K57A) (TAOK1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed K 57 to A

Promoter

CMV

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax

Plasmid

#190154

Purpose

Expresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp3 (Vamp2 Rat)

Use

AAV

Tags

P2AT2A-EGFP-caax

Expression

Mammalian

Mutation

Truncation that includes only rat Vamp3 AA #1-81

Promoter

MBP

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEE055

Plasmid

#176812

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

606

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hSTK16-Flag

Plasmid

#180403

Purpose

lentiviral transduction of human STK16 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

STK16 (STK16 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_Flag-hSRPK3

Plasmid

#180404

Purpose

lentiviral transduction of human SRPK3 (with an N-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

SRPK3 (SRPK3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15541_dCBE-SuperFi-Cas9

Plasmid

#184371

Purpose

Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

dCBE-SuperFi-Cas9

Use

CRISPR

Tags

3X FLAG, SV40 NLS, and UGI

Expression

Mammalian

Mutation

D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15543_dABE-SuperFi-Cas9

Plasmid

#184373

Purpose

Mammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

dABE-SuperFi-Cas9

Use

CRISPR

Tags

FLAG, SV40 NLS, and nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15545_dABE8e-SuperFi-Cas9

Plasmid

#184375

Purpose

Mammalian expression of nuclease inactive SuperFi-Cas9 ABE8e base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

dABE8e-SuperFi-Cas9

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Mutation

D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Rac-DN(guide resistant)-2a-mcherry-U6ac-Rac guides

Plasmid

#168243

Purpose

"Dominant negative control of the assay to rescue Rac expression in neutrophil specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Rac(guide resistant)-DN

Use

Zebrafish expression

Tags

mcherry

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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