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pME_G_0_1_022_5C4SF

Plasmid

#235792

Purpose

5' Connector 4 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 4 Short Forward

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1_014_5C5SF

Plasmid

#235793

Purpose

5' Connector 5 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 5 Short Forward

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1_005_5C1SF

Plasmid

#235789

Purpose

5' Connector 1 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 1 Short Forward

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_7-8_004_Amp/ColEI_sfGFP

Plasmid

#235970

Purpose

Ampicillin/ColEI backbone with sfGFP placeholder for lvl1 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Amp/ColEI

Use

Synthetic Biology

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mTSP15

Plasmid

#236542

Purpose

For expression of Tspan15 in mammalian cells

Depositor

Sandra Citi

Article

Shah et al Cell Rep. 2018 Nov 20;25(8):2132-2147.e7

Insert

TSPAN15 (Tspan15 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

WT

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 mR0-37a +SV40 base prom-GFP-IRES-AP

Plasmid

#225889

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

mR0-37a

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 mR3-17c +SV40 base prom-GFP-IRES-AP

Plasmid

#225890

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

mR3-17c

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 mR3-17d +SV40 base prom-GFP-IRES-AP

Plasmid

#225891

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

mR3-17d

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 Mini-enhancer +SV40 base prom-GFP-IRES-AP

Plasmid

#225893

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

VSX2 Mini-enhancer

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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1010Av2

Plasmid

#125642

Purpose

Expresses FAR protein under 10xUAS control

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

FAR protein

Expression

Insect

Promoter

10xUAS

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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1010Bv2

Plasmid

#125643

Purpose

Expresses FAR protein under 10xUAS control

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

FAR protein

Expression

Insect

Promoter

10xUAS

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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Dendra2-NS2B

Plasmid

#204218

Purpose

Expresses Dendra2-NS2B in Mammalian / Mouse Cell Lines

Depositor

Partha Mondal

Article

Aravinth et al Sci Rep. 2025 Mar 26;15(1):10358. doi: 1

Insert

Dengue (Dengue Type 2) NS2B (POLY Dengue Virus Serotype , Strain NGC)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mBrn2-P2A-mRuby2

Plasmid

#233156

Purpose

Retroviral expression of fluorescent mRuby2 reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Brn2-2A-mRuby2 (Pou3f2 Mouse, Synthetic)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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inducible shSRP19_9 in pLKO tet on

Plasmid

#233289

Purpose

Dox inducible expression of SRP19 shRNA

Depositor

Sefi Rosenbluh

Article

Xi et al Proc Natl Acad Sci U S A. 2025 Apr 15;12

Insert

shSRP19

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

Dox

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-NT-BTy

Plasmid

#232206

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

3xTy

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-PTy

Plasmid

#232203

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-PAC-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

3xTy

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-BTy

Plasmid

#232209

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-BSD-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

3xTy

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-NT-PTy

Plasmid

#232202

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

3xTy

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCas9-EcRT-Z3-Kan

Plasmid

#232096

Purpose

Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

Z3 promoter and Z3EV transcription factor

Use

CRISPR

Expression

Yeast

Promoter

Z3

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pIND-GLucZ

Plasmid

#228704

Purpose

Inducible expression of Gaussia luciferase and shRNAmir of interest

Depositor

Thorsten Stiewe

Article

Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

Inserts

Gaussia luciferase
non-silencing shRNA

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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