We narrowed to 150,751 results for: ins

1. pCarCBADE_ProB(I69E)A

   Plasmid

   #117756

   Purpose

   ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone

   Depositor

   Greg Bokinsky

   Article

   Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

   Insert

   Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
   

   Expression

   Bacterial

   Promoter

   PLac(UV5)

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CaMKIIa-hChR2(E123A)-mCherry

   Plasmid

   #35506

   Purpose

   AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E123A

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD

   Plasmid

   #168153

   Purpose

   Inducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   PmcCAST Tns proteins (TnsAB and TnsC)
   PmcTniQ
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX Stat5A-SH2

   Plasmid

   #46515

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Stat5A (STAT5A Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 582-693

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV5 MEKK4alpha WT

   Plasmid

   #12187

   Depositor

   Gary Johnson

   Article

   Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

   Insert

   MEKK4 (Map3k4 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pLKO.shHmga2

   Plasmid

   #32399

   Depositor

   Tyler Jacks, Monte Winslow

   Article

   Winslow et al Nature. 2011 May 5. 473(7345):101-4.

   Insert

   shRNA targetting Hmga2 (Hmga2 Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+PhyBΔCreC

   Plasmid

   #131781

   Purpose

   Mammalian, Zebrafish, or Xenopus expression of PhyBΔCreC, one half of the CreLite system.

   Depositor

   George Eisenhoffer

   Article

   Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

   Insert

   PhyBCreC
   

   Use

   Cre/Lox; Zebrafish expression

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX Stat5B-SH2

   Plasmid

   #46516

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Stat5B (STAT5B Human)
   

   Tags

   AviTag, GST, and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 593-692

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nrxn3α-AS4+

   Plasmid

   #184333

   Purpose

   Expression of mouse Nrx3-alpha containing insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

   Depositor

   Peter Scheiffele

   Article

   Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife

   Insert

   Nrxn3-alpha (Nrxn3 Mouse)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR4-huNFATc1nuc

    Plasmid

    #23988

    Depositor

    Jerry Crabtree

    Article

    Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

    Insert

    NFATc1 (NFATC1 Human)
    

    Use

    Cloning vector

    Tags

    EE tag

    Mutation

    S172A S175A S176A S178A S179A S181A S184A S187A S…

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-CaMKIIa-eArch 3.0-EYFP

    Plasmid

    #35514

    Purpose

    Lentiviral expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    eArch3.0-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_GWRev_Dest

    Plasmid

    #86954

    Purpose

    Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. With cre, insert will flip backwards, causing it to turn off (cre-off)

    Depositor

    Joseph Dougherty

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSF-G1(6CNW)RS

    Plasmid

    #222873

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV_GW_Dest

    Plasmid

    #86955

    Purpose

    Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. Without cre, insert is backwards in reference to promoter.

    Depositor

    Joseph Dougherty

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX ShcD-SH2

    Plasmid

    #46488

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    ShcD (SHC4 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 512-623

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX SH2-B-SH2

    Plasmid

    #46479

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SH2-B (SH2B1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 515-639

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET a MatryoshCaMP6s

    Plasmid

    #100019

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    MatryoshCaMP6s
    

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-2)

    Plasmid

    #159660

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-3)

    Plasmid

    #159661

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1)

    Plasmid

    #159659

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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