We narrowed to 47,111 results for: Nes

1. pETM6-RgTALsyn

   Plasmid

   #100947

   Purpose

   Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Insert

   RgTALsyn
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUB11

   Plasmid

   #169699

   Purpose

   Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

   Depositor

   Nara Figueroa

   Article

   Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

   Insert

   3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
   

   Use

   Tags

   3xFLAG tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR221-NR1H3

   Plasmid

   #79514

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   LXR Alpha (NR1H3 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-29b-3xGluGlu-c-jun-His6

   Plasmid

   #129227

   Purpose

   Encodes 3xGluGlu tagged human c-Jun for purification by nickel affinity

   Depositor

   Kevin Janes

   Article

   Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

   Insert

   cJun (JUN Human)
   

   Use

   Tags

   6xHistidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSUB7

   Plasmid

   #169698

   Purpose

   Template plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.

   Depositor

   Nara Figueroa

   Article

   Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

   Insert

   6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-3xflagGREB1a

   Plasmid

   #112221

   Purpose

   mammalian expression of flag tagged GREB1a

   Depositor

   Craig Burd

   Article

   Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

   Insert

   GREB1a (GREB1 Human)
   

   Use

   Tags

   3x flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEC3079 (p7INT-Pveg_lacI)

   Plasmid

   #218505

   Purpose

   integrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   lacI (lacI Escherichia coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pveg

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSU315

   Plasmid

   #169697

   Purpose

   Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.

   Depositor

   Nara Figueroa

   Article

   Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

   Insert

   1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
   

   Use

   Tags

   1xHA tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. G-GECO1-Orai3

   Plasmid

   #73563

   Purpose

   Green fluorescent reporter of Orai3-associated calcium influx

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   G-GECO1-Orai3 (ORAI3 Human, Synthetic)
   

   Use

   Tags

   G-GECO1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Immdeiate Early

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. μ2-HA-D176A

    Plasmid

    #32754

    Depositor

    Alexander Sorkin

    Article

    Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

    Insert

    μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D176A

    Promoter

    CMV

    Available Since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pLX302 OAS2-V5 puro

    Plasmid

    #158642

    Purpose

    Lentiviral expression vector for constitutive expression of V5-tagged OAS2.

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    OAS2 (OAS2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330A_D10A-1x5

    Plasmid

    #58775

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJT9R

    Plasmid

    #98305

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    TRPL3AcNES1>TRPL41B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Rap1A FLARE

    Plasmid

    #134926

    Purpose

    Rap1A biosensor

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    Rap1A, mCerulean3, RaIGDS RBD, YPet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Rap1B FLARE

    Plasmid

    #134927

    Purpose

    Rap1B biosensor

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    RaIGDS RBD, YPet, Cerulean, Rap1B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKMD106e - intein-Cas9(S219)

    Plasmid

    #64190

    Purpose

    Expresses intein-Cas9(S219) in mammalian cells

    Depositor

    David Liu

    Article

    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

    Insert

    mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG
    

    Use

    CRISPR

    Tags

    NLS - 3x FLAG (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)

    Plasmid

    #179005

    Purpose

    Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)

    Plasmid

    #179004

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBa.Kif5c 1-559.GFP

    Plasmid

    #45059

    Depositor

    Marvin Bentley, Gary Banker

    Article

    Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

    Insert

    Kif5c (Kif5c Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    motor domain aa1-559

    Promoter

    Chicken Beta Actin

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. UAS-u(M)Cas9

    Plasmid

    #127384

    Purpose

    Expresses Cas9 at medium level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(M)-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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