We narrowed to 11,078 results for: 158

1. pLKO_Ollas.C.FLAG_NGFR

   Plasmid

   #158311

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Ollas.C.FLAG

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO_Ollas.C.NWS_NGFR

   Plasmid

   #158312

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Ollas.C.NWS

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO_Ollas.C.HA_NGFR

   Plasmid

   #158313

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Ollas.C.HA

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCTCON2-BXG-2TAG

   Plasmid

   #158138

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   BFP-GFP fragment
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Cloned in a TAG codon at the first serine residue…

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHCKAn-yibDp-GRFT

   Plasmid

   #158745

   Purpose

   low phosphate yibDp induction of Griffithsin

   Depositor

   Michael Lynch

   Article

   Decker et al Front Bioeng Biotechnol. 2020 Aug 21;8:1

   Insert

   yibDp Griffithsin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibD

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO_HA.VSVg.C_NGFR

   Plasmid

   #158263

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   HA.VSVg.C

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO_FLAG.VSVg.C_NGFR

   Plasmid

   #158260

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG.VSVg.C

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMOR28-1-ISH

   Plasmid

   #15843

   Depositor

   Peter Mombaerts

   Article

   Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

   Insert

   MOR28-1 (Olfr552 Mouse)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pJAS1669

   Plasmid

   #158594

   Purpose

   VioE-dead_ePTS1 CEN6/ARS4

   Depositor

   John Dueber

   Article

   Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

   Insert

   VioE-dead_ePTS1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 2xFLAG-2xSTREP EMI1

    Plasmid

    #158947

    Purpose

    Express human EMI1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    EMI1 (FBXO5 Human)
    

    Use

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p663-UBC-miniTurbo-V5-GSK3B_IDG-K

    Plasmid

    #158165

    Purpose

    Gateway destination clone of GSK3B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-GSK3B (GSK3B Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pdCas9- AsiA_m1.1

    Plasmid

    #158064

    Purpose

    Expressing evolved CasTA of dCas9 fused AsiA variant 1.1

    Depositor

    Harris Wang

    Article

    Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

    Insert

    dCas9-AsiA_m1.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZA27

    Plasmid

    #158505

    Purpose

    In planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-4xMyc. Km plant selection marker.

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1-4xMyc (ZAR1 Mustard Weed)
    

    Use

    Tags

    4xMyc

    Expression

    Plant

    Mutation

    L17E

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO-Cre sgAdam10 v3

    Plasmid

    #158034

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Adam10

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Adam10 sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pF(UG) hSyn SYP-SELF

    Plasmid

    #158770

    Purpose

    Expression of synaptophysin 1 variant with self-cleaving c-terminal tail.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    Synaptophysin 1-NS3/4a (Syt1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Flexible linkers, ADVVCC/QMSY cleavage site

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMOR21-1-ISH

    Plasmid

    #15838

    Depositor

    Peter Mombaerts

    Article

    Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

    Insert

    MOR21-1 (Olfr571 Mouse)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pSI-356 v2

    Plasmid

    #158431

    Purpose

    SpCas9 sgRNA cloning backbone

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Methods Mol Biol. 2021;2312:193-223. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human H6

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pISH-bovine-tau

    Plasmid

    #15864

    Depositor

    Peter Mombaerts

    Article

    Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

    Insert

    bovine tau (MAPT )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pAAV_2pabPAC

    Plasmid

    #158969

    Purpose

    2P-activatable bPAC for AAV

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

    Insert

    2pabPAC
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Sticky-147-4X

    Plasmid

    #158575

    Purpose

    Production of DNA fragment for nucleosome core particle preparation

    Depositor

    Curt Davey

    Article

    Sharma et al Nat Commun. 2019 Dec 17;10(1):5751. doi:

    Type

    Empty backbone

    Use

    Bacterial vector for dna production

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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