-
Plasmid#116968PurposeExpresses CaSK-02RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertCandidatus Accumulibacter sp. SK-02 RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0355_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._SK-02_ARRAY_1
Plasmid#116967PurposeExpresses CaSK-02RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertCandidatus Accumulibacter sp. SK-02 RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0348_pET30-RT-CRISPR_Campylobacter_fetus_subsp._Fetus_ARRAY_1_RC
Plasmid#116960PurposeExpresses CfRT-Cas1-Cas2 under pT7lac promoter, encodes CfCRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertCampylobacter fetus subsp. Fetus RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pISH-Pou2f1
Plasmid#74355PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Pou2f1. Not for gene expression.DepositorInsertPOU domain, class 2, transcription factor 1 (Pou2f1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5 c-Fyn
Plasmid#16032DepositorInsertc-Fyn (FYN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Cortactin
Plasmid#74476PurposeThis plasmid was constructed to study cortactin interactionsDepositorInsertCortactin (Cttn Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCLXEBR-pTF-Ngn3
Plasmid#114307PurposeTET-inducible expression of the Neugenin 3 transcription factorDepositorInsertNgn3 (NEUROG3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJP-HBB-nLuc
Plasmid#175431PurposeExpresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.DepositorInsertnLUC plus 5' HBB leader
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXG1
Plasmid#153124PurposeExpression of Flag tagged human FOXG1 in mammalian cellsDepositorInserthuman FOXG1 (FOXG1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5 DN-Fyn
Plasmid#16033DepositorInsertc-Fyn (FYN Human)
UseTagsExpressionMammalianMutationDominant negative: K299MPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
EK0493 SFFV-mCherry-SGc(GAPDH.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191156PurposeExpression of a sensor for GAPDH 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTRDepositorInsertmCherry:GAPDH.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX nsp1 CoV2
Plasmid#175512PurposeFor protein expression of WT nsp1 CoV2DepositorInsertnsp1 CoV2
UseTagsGST tagExpressionBacterialMutationPromotertac promoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
EK0445 SFFV-mCherry-SGc(DNAJB1.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191155PurposeExpression of a sensor for human DNAJB1 (hsp40) 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTRDepositorInsertmCherry:DNAJB1.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
EC71167
Plasmid#154066PurposeLevel 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFPDepositorInsertZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
UseSynthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterZea mays Ubiquitin PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
EK0379 CMV-TO-EGFP-stop-t70587 (PB)
Plasmid#191154PurposeExpression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.DepositorInsertEGFP-t70587
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprt
Plasmid#92166PurposePlasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vectorDepositorInsertQuinolinate Phosphoribosyltransferase (QPRT Human)
UseTagsTEV protease -cleavable N-terminal 6HisExpressionBacterialMutationchanged Asp 2 to Asn to create BspHI sitePromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMaKIIalpha-mKO2
Plasmid#86156PurposeLentiviral fluorescent reporter using the neuronal cell type-specific promoter of the CaM Kinase II alpha gene.DepositorInsertmKO2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMU103
Plasmid#73934PurposeThis is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.DepositorInsertbar and GUS cassettes
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -