Addgene

We narrowed to 1,206 results for: Hras;

Showing: 621 - 640 of 1206 results

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pALOD4 non-binding mutant

Plasmid

#111027

Purpose

Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)

Depositor

Arun Radhakrishnan

Article

Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

Insert

Domain 4 of Anthrolysin O

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: S404C, C472A, G501A, T502A, T503A, L5…

Promoter

T7

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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WT (REP10)

Plasmid

#21368

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pECIA2

Plasmid

#47032

Purpose

This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.

Depositor

Chris Garcia

Article

Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

Type

Empty backbone

Use

Secreted expression in drosophila culture

Tags

Fc(human IgG1), HRV 3C Protease Cleavage site, He…

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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p1071-Lenti-Single loxP

Plasmid

#217892

Purpose

Lentiviral vector, entry vector for Lentiviral Switch-OVER

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5A-CA-CMV-GFP

Plasmid

#83255

Purpose

Adenoviral expression of constitutively active STAT5A with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5a (Stat5a Mouse)
eGFP

Use

Adenoviral

Tags

Expression

Mutation

constitutively active

Promoter

CMV

Available since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-VARNAM2

Plasmid

#195520

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2

Use

AAV

Tags

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

Synapsin

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-UMI

Plasmid

#222694

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

PGK without BsmBI cutsite

Promoter

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5A-CMV-GFP

Plasmid

#83251

Purpose

Adenoviral expression of STAT5A with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5a (Stat5a Mouse)
eGFP

Use

Adenoviral

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pQTEV-CDO1

Plasmid

#31292

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

cysteine dioxygenase, type I (CDO1 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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p944-SWITCH-OVER insert: EF1a-Puro

Plasmid

#217891

Purpose

insert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5B-CMV-GFP

Plasmid

#83257

Purpose

Adenoviral expression of STAT5B with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5b (Stat5b Mouse)
eGFP

Use

Adenoviral

Tags

Expression

Mutation

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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WT (AF20)

Plasmid

#21359

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Promoter

Available since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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pCW57.1-ZNF746

Plasmid

#207825

Purpose

Dox-inducible expression of ZNF746

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746 (ZNF746 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe-Sik-WT

Plasmid

#210722

Purpose

Construct for stable expression of mouse wild type Ptk6 (Sik) in mammalian cells.

Depositor

Angela Tyner

Article

Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

Insert

Protein tyrosine kinase 6 (Ptk6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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HM3C NaVAe1_p pET24b

Plasmid

#127854

Purpose

Bacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVAe1p

Use

Tags

3C Cleavage site, His, and MBP

Expression

Bacterial

Mutation

Promoter

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pQTEV-GYG

Plasmid

#31324

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

glycogenin (GYG1 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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p413TEF-SirT1

Plasmid

#51746

Purpose

overexpression of human SirT1 in S. cerevisiae, HIS3 marker

Depositor

Markus Ralser

Article

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

SirT1 (SIRT1 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pQStrep2-CRYM

Plasmid

#31293

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

crystallin, mu (CRYM Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pQTEV-UFD1L

Plasmid

#31560

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

ubiquitin fusion degradation 1-like (UFD1 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pQTEV-COPE

Plasmid

#31585

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

coatomer protein complex, subunit epsilon (COPE Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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