We narrowed to 4,744 results for: NAP

1. pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby

   Plasmid

   #71760

   Purpose

   mGFP and Synaptophysin-mRuby expression from Cre-expressing neurons

   Depositor

   Liqun Luo

   Article

   Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

   Has Service

   AAV1

   Inserts

   mGFP
   Synaptophysin
   

   Use

   AAV and Cre/Lox

   Tags

   Palmitoylation signal and mRuby

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20945

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   hSyn

   Available Since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

   Plasmid

   #182653

   Purpose

   Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   mutant eukaryotic release factor 1
   

   Use

   Tags

   HA tag and nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   E55D and Y306A/Y384F (AF) double mutant of Methan…

   Promoter

   CMV and U6

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO_CalR-SNAP-tag2-KDEL

   Plasmid

   #226517

   Purpose

   Mamalian cell expression of SNAP-tag2 in endoplasmic reticulum

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   CalR-SNAP-tag2-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-LoxP-PURO-LoxP-SNAP-TERT

   Plasmid

   #71390

   Purpose

   Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.

   Depositor

   Thomas Cech

   Article

   Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

   Insert

   TERT (TERT Human)
   

   Use

   Hr donor

   Tags

   FLAG-SNAP

   Expression

   Mutation

   Promoter

   Endogenous TERT promotor

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3

   Plasmid

   #112005

   Purpose

   For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Has Service

   AAV5 and AAV9

   Insert

   axon-GCaMP6s-P2A-mRuby3
   

   Use

   AAV

   Tags

   GAP43 palmitoylation domain

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Synapsin sHRPa-NRX in FSW lentiviral vector

   Plasmid

   #73147

   Purpose

   Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPa-NRX
   

   Use

   Lentiviral

   Tags

   2x HA tag and Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3

   Plasmid

   #172922

   Purpose

   For enriched expression of jGCaMP8m in axons with cytosolic expression of mRuby3

   Depositor

   GENIE Project

   Has Service

   AAV1 and AAV9

   Insert

   axon-jGCaMP8m-P2A-mRuby3
   

   Use

   AAV

   Tags

   GAP43 palmitoylation domain (N terminal on insert…

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby

   Plasmid

   #71761

   Purpose

   mGFP and Synaptophysin-mRuby expression from Flp-expressing neurons

   Depositor

   Liqun Luo

   Article

   Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

   Inserts

   mGFP
   Synaptophysin
   

   Use

   AAV

   Tags

   Palmitoylation signal and mRuby

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLAG-LoxP-GFP-LoxP-SNAP-TERT

    Plasmid

    #71391

    Purpose

    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Use

    Hr donor

    Tags

    FLAG-SNAP

    Expression

    Mutation

    Promoter

    Endogenous TERT promotor

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. loxP-Puro-loxP-3xV5-SNAP-Rab11 HR

    Plasmid

    #229677

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    SNAP, 3xV5

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2

    Plasmid

    #112004

    Purpose

    For enriched expression of GCaMP6s in axons with cytosolic expression of mKate2

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV9

    Insert

    axon-GCaMP6s-P2A-mKate2
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Synapsin sHRPb-NLG in FSW lentiviral vector

    Plasmid

    #73148

    Purpose

    Small fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    sHRPb-NLG (Nlgn1 Mouse, Synthetic)
    

    Use

    Lentiviral

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-NLS-ddMisCas13b-SNAPf-NLS-3xFlag

    Plasmid

    #191537

    Purpose

    Overexpression of NLS-ddMisCas13b-SNAPf-NLS-3xFlag in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-ddMisCas13b-SNAPf-NLS-3xFlag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre

    Plasmid

    #134308

    Purpose

    Expresses Synaptophysin-TdTomato and also tamoxifen dependant Cre (OHT-Cre, aka Cre-ER)

    Depositor

    Elly Nedivi

    Article

    Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

    Inserts

    Synaptophysin (Syp Rat, Mouse)
    Cre-ER
    

    Use

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-NLS-dMisCas13b-SNAPf-NLS-3xFlag

    Plasmid

    #191536

    Purpose

    Overexpression of NLS-dMisCas13b-SNAPf-NLS-3xFlag in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-dMisCas13b-SNAPf-NLS-3xFlag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3

    Plasmid

    #172921

    Purpose

    For enriched expression of jGCaMP8s in axons with cytosolic expression of mRuby3

    Depositor

    GENIE Project

    Has Service

    AAV1 and AAV9

    Insert

    axon-jGCaMP8s-P2A-mRuby3
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain and P2A-mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDH-EF1-TfR20-SNAP-IRES-Puro

    Plasmid

    #171018

    Purpose

    Lentiviral expression of a transferrin receptor-SNAP-tag fusion in mammalian cells

    Depositor

    Angus Johnston

    Article

    FitzGerald et al Nat Commun. 2020 Sep 8;11(1):4482. doi:

    Insert

    Transferrin receptor (Human) - SNAP-tag (TFRC Human)
    

    Use

    Lentiviral

    Tags

    SNAPtag and TfR

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)

    Plasmid

    #187844

    Purpose

    Expression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    IFNAR1 (IFNAR1 Human)
    

    Use

    Tags

    Ig k-chain leader sequence, SNAPf-tag, and meGFPe…

    Expression

    Mammalian

    Mutation

    meGFPe: asparagine 198 to aspartic acid and tyros…

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3

    Plasmid

    #172923

    Purpose

    For enriched expression of jGCaMP8f in axons with cytosolic expression of mRuby3

    Depositor

    GENIE Project

    Has Service

    AAV1 and AAV9

    Insert

    axon-jGCaMP8f-P2A-mRuby3
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain (N terminal on insert…

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge