We narrowed to 32,671 results for: eng

1. pHYX138

   Plasmid

   #202815

   Purpose

   Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.

   Depositor

   Jean-Félix Dallery

   Article

   Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   ScADH2

   Available Since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS2374-Tier1-PhCMV-Gal4_VP16

   Plasmid

   #169582

   Purpose

   Tier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   GAL4-transactivator
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPMVAd3

   Plasmid

   #98298

   Purpose

   Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

   Insert

   PRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
   

   Expression

   Yeast

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

   Plasmid

   #83947

   Purpose

   gRNA cassette-carrying vector with natMX marker

   Depositor

   Irina Borodina

   Article

   Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

   Insert

   gRNA targeting ADE2 gene
   

   Expression

   Yeast

   Available Since

   Oct. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPPC019.AAV

   Plasmid

   #171147

   Purpose

   Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   hAAVS1 scRNA
   J1-BBa_J23117-mRFP
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119 and J1-BBa_J23117

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVH64-Tier1-PSV40-Citrine_p2a_SEAP

   Plasmid

   #169558

   Purpose

   Tier-1 vector encoding PSV40-driven Citrine-p2A-SEAP expression (PSV40-Citrine-p2A-SEAP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PSV40-driven citrine-P2A-SEAP fusion construct
   

   Expression

   Mammalian

   Promoter

   PSV40

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPPC005

   Plasmid

   #171140

   Purpose

   For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   XylS-Pm
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   Pm

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. NFkBr-IL1Ra

   Plasmid

   #183065

   Purpose

   Expresses IL-1Ra downstream of a synthetic promoter activated by the NFkB signaling pathway

   Depositor

   Farshid Guilak

   Article

   Pferdehirt et al Tissue Eng Part A. 2019 May;25(9-10):809

   Insert

   interleukin-1 receptor antagonist (Il1rn Mouse)
   

   Use

   Lentiviral

   Available Since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas9-beta

   Plasmid

   #169240

   Purpose

   Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Inserts

   cas9
   beta
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas34

    Plasmid

    #82386

    Purpose

    sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PEZ3

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHB-2

    Plasmid

    #140955

    Purpose

    For PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.

    Depositor

    Kang Zhou

    Article

    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

    Insert

    phaCAB
    

    Expression

    Bacterial

    Mutation

    R238S mutation in PhaC

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCOLA-Spec-yibDp-Erv1p

    Plasmid

    #212094

    Purpose

    Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    Sulfydryl oxidase (ERV1 Budding Yeast)
    

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPMVAu5 (R)

    Plasmid

    #98296

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    PSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
    

    Expression

    Yeast

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS2386-Tier1-PhCMV-GCaMP6s

    Plasmid

    #169519

    Purpose

    Tier-1 vector encoding PhCMV-driven calcium sensor GCaMP6s (PhCMV-GCaMP6s-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven green fluorescent genetically encoded calcium indicator GCaMP6s
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCK255

    Plasmid

    #171155

    Purpose

    Expression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    I-SceI_sacB
    

    Expression

    Bacterial

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM6-Amp-T7-roGFP

    Plasmid

    #202464

    Purpose

    Expresses redox sensitive GFP (roGFP) with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    reduction-oxidation sensitive green fluorescent protein
    

    Tags

    6X-His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS2365-Tier1-PhCMV-TetR

    Plasmid

    #169566

    Purpose

    Tier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven tetracycline repressor protein class B from transposon Tn10
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCOLA-Gent-yibDp-Erv1p

    Plasmid

    #202483

    Purpose

    Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
    

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTS2396-Tier1-PhCMV-4xCOX8_Ypet

    Plasmid

    #169534

    Purpose

    Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven miochondria-localized yellow fluorescent protein Ypet
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pgRNAtet-phaZ

    Plasmid

    #169874

    Purpose

    Single guide RNA plasmid for Cas9 targeting phaZ in P. putida

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    sgRNA towards phaZ in Pseudomonas putida
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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