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pXD80ENSF7.7-Dadh-ΔElen0477(ElA2)

Plasmid

#225322

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0477) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0477)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.8-Dadh-ΔElen0478(ElA2)

Plasmid

#225323

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0478) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0478)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.9-Dadh-ΔElen0481(ElA2)

Plasmid

#225324

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0481) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0481)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.10-Dadh-ΔElen0482(ElA2)

Plasmid

#225325

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0482) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0482)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70DA8-DadhA(cHis8)BC-DadRS-noAccs(ElA2)

Plasmid

#225326

Purpose

Expresses Eggerthella lenta A2 Dadh in Eggerthella lenta DSM 2243

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7-DadhA(cHis8)BC-DadRS-Accs(ElA2)

Plasmid

#225302

Purpose

Expresses Eggerthella lenta A2 Dadh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF8-HcdhA(cHis8)BC-HcdR-Accs(ElA2)

Plasmid

#225303

Purpose

Expresses Eggerthella lenta A2 Hcdh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Hcdh

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN3-Elen0507-Cadh-noAccs(ElA2)

Plasmid

#225311

Purpose

Expresses Eggerthella lenta A2 Cadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Cadh

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN4-Elen0511-(–)-Eldh1-noAccs(ElA2)

Plasmid

#225312

Purpose

Expresses Eggerthella lenta A2 (–)-Eldh1 without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh1 (Elen_0511 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF3-Elen0507-Cadh-Accs(ElA2)

Plasmid

#225298

Purpose

Expresses Eggerthella lenta A2 Cadh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Cadh

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD70EN25-Pct5-(–)-Eddh(Gp3C)

Plasmid

#233877

Purpose

Expresses Gordonibacter pamelaeae 3C (–)-Eddh in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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PtetA with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225644

Purpose

tetA promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

tetR/tetA

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

tetA promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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PLac0301 with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225643

Purpose

Lac0301 promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

LacO3O1

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

LacO3O1 promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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ParaBAD with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225642

Purpose

araBAD promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

araBAD

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

BAD promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pO5

Plasmid

#193749

Purpose

Expresses tdTomato under the control of PIAH synthetic promoter regulated by AHL and IPTG, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al ACS Synth Biol. 2021 Oct 15;10(10):2456-

Insert

tdTomato

Use

Tags

Expression

Bacterial

Mutation

Promoter

PIAH

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pO3

Plasmid

#193748

Purpose

Expresses E2-Crimson under the control of PAAH synthetic promoter regulated by AHL and aTc, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al ACS Synth Biol. 2021 Oct 15;10(10):2456-

Insert

E2-Crimson

Use

Tags

Expression

Bacterial

Mutation

Promoter

PAAH

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pO6

Plasmid

#193747

Purpose

Expresses E2-Crimson under the control of PIAH synthetic promoter regulated by AHL and IPTG, p15A origin of replication, Chloramphenicol selection,

Depositor

Sangram Bagh

Article

Sarkar et al ACS Synth Biol. 2021 Oct 15;10(10):2456-

Insert

E2-Crimson

Use

Tags

Expression

Bacterial

Mutation

Promoter

PIAH

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pTC3RBSC1cI

Plasmid

#193773

Purpose

Expresses lambda CI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

Lambda CI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLTetO-1

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pX*C3E2-Crimson

Plasmid

#193754

Purpose

Expresses E2-Crimson under the control of PLUX* promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

E2-Crimson

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLUX*

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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PRC3MKO2

Plasmid

#193757

Purpose

Expresses mKO2 under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

mKO2

Use

Tags

Expression

Bacterial

Mutation

Promoter

PR

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge