We narrowed to 150,751 results for: ins

1. HOXA10-MSCV

   Plasmid

   #20980

   Depositor

   Corey Largman

   Article

   Thorsteinsdottir et al Mol Cell Biol. 1997 Jan . 17(1):495-505.

   Insert

   HOXA10 (HOXA10 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pEAK-cKal7

   Plasmid

   #145801

   Purpose

   expresses Kalirin-7 with the c-front in mammalian cells

   Depositor

   Betty Eipper, Richard Mains

   Article

   Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

   Insert

   cKalirin-7 (Kalrn Rat)
   

   Tags

   none

   Expression

   Mammalian

   Promoter

   EIF2a

   Available Since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CreLite

   Plasmid

   #131786

   Purpose

   Lentiviral vector expressing CreLite, driven by CBh promoter

   Depositor

   George Eisenhoffer

   Article

   Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

   Insert

   PhyBCreC-P2A-PIF6CreN
   

   Use

   Cre/Lox, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pINDgw RFA-V5-EcoDam

   Plasmid

   #59204

   Purpose

   Mammalian DamID vector for fusing gene of interest with Dam-V5 using Gateway cloning

   Depositor

   Bas van Steensel

   Article

   Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

   Type

   Empty backbone

   Use

   Damid

   Tags

   Dam (DNA adenine methyltransferase) and V5

   Expression

   Mammalian

   Promoter

   Heat Shock Minimal Promoter

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV hSyn1 ChR2 ET-TC 2A tDimer

   Plasmid

   #101361

   Purpose

   High efficiency channelrhodopsin, neuron specific promoter, red fluorescent protein

   Depositor

   Thomas Oertner

   Article

   Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

   Inserts

   Channelrhodopsin-2
   synaptophysin-red fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   E123T , T159C

   Promoter

   human Synapsin

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX Yes-SH2

   Plasmid

   #46532

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Yes (YES1 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 152-261

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-STChRger2-TS-EYFP

   Plasmid

   #129395

   Purpose

   Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   soma targeted ChRger2
   

   Use

   AAV

   Tags

   Kv2.1-TS-EYFP

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

   Plasmid

   #115137

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS

   Depositor

   Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA2.1 anti-CRISPR protein
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMS23: pHelper(PmcCAST)_entry_ΔTnsD

   Plasmid

   #168156

   Purpose

   Inducible expression of PmcCAST proteins (except TnsD). Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   PmcCAST Tns proteins (TnsAB and TnsC)
   PmcTniQ
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. ZIKV_Trigger_32B

    Plasmid

    #75009

    Purpose

    Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequence

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_Trigger_32B
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI.neo-rPAM1-M314I

    Plasmid

    #145802

    Purpose

    expresses catalytically inactive rat PAM-1 in mammalian cells

    Depositor

    Betty Eipper, Richard Mains

    Article

    Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.

    Insert

    rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
    

    Expression

    Mammalian

    Mutation

    changed methionine 314 to isoleucine to inactivat…

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX Txk-SH2

    Plasmid

    #46526

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Txk (Txk Mouse)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 138-258

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. VGFR1_HUMAN_D0

    Plasmid

    #79743

    Purpose

    This plasmid encodes the kinase domain of VGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    VGFR1
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pDEST8_FLAGHA_FUS_HIS

    Plasmid

    #26375

    Depositor

    Thomas Tuschl

    Article

    Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

    Insert

    FUS (FUS Human)
    

    Tags

    FLAG/HA and His

    Expression

    Insect

    Mutation

    delta 527 (STOP codon)

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

15. AKT-APEX2-P2A-mKate2

    Plasmid

    #67654

    Purpose

    Mammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    AKT-PH-APEX2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSP2372

    Plasmid

    #73196

    Purpose

    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A, Q695A, and Q926A in SpCas9

    Promoter

    CMV

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHS1068

    Plasmid

    #205962

    Purpose

    NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    DinG HNH proteins and CRISPR array
    

    Expression

    Bacterial

    Promoter

    Lac (proteins), pJ23119 (crRNA)

    Available Since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-GP-2-DNMT3A PWWP

    Plasmid

    #59696

    Purpose

    Contains histone modification interacting domain (DNMT3A PWWP)

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

    Insert

    DNMT3A PWWP (Dnmt3a Mouse)
    

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBacDual with LFn + FlaAAA

    Plasmid

    #84867

    Purpose

    Baculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    FlaAAA
    

    Use

    Baculovirus

    Tags

    6xHis

    Expression

    Insect

    Mutation

    3 C-terminal leucines have been replaced by alani…

    Promoter

    Polyhedrin

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX Sh3bp2-SH2

    Plasmid

    #46484

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Sh3bp2 (SH3BP2 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 450-561

    Promoter

    Tac

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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