We narrowed to 7,293 results for: Ank

1. pAV10.F3

   Plasmid

   #63199

   Purpose

   BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-MLC2(CA)-IRES-GFP

   Plasmid

   #133928

   Purpose

   Bicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain

   Depositor

   Frank Bradke, Sebastián Dupraz

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   EGFP and MLC2(CA) (MYL2 Human)
   

   Expression

   Mammalian

   Mutation

   MLC2 T18D, S19D

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH3mCHSH2

   Plasmid

   #101053

   Purpose

   This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.

   Depositor

   Jennifer Lodge

   Article

   Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108

   Insert

   Cryptococcus mCherry expression plasmid
   

   Expression

   Yeast

   Promoter

   Cryptococcus Histone3 promoter

   Available Since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSKC11 - UTRQ

   Plasmid

   #110490

   Purpose

   Monitor the expression of Luciferase driven by the NRAS 5'-UTR.

   Depositor

   Shankar Balasubramanian

   Article

   Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

   Insert

   NRAS 5'-UTR (bp 1-254) (NRAS Human)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Promoter

   T7

   Available Since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. RDX-mCherry

   Plasmid

   #214476

   Purpose

   Plasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.

   Depositor

   Natalie Ahn

   Article

   Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

   Insert

   RDX (RDX Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Sequence matches cDNA clone MGC:48283 IMAGE:52844…

   Promoter

   CMV

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3015b

   Plasmid

   #209317

   Purpose

   Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

   Depositor

   Thomas Hooven

   Article

   Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

   Insert

   Group B Streptococcus-compatible single guide RNA
   

   Expression

   Bacterial

   Promoter

   Xyl/tet promoter that is constitutively active

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-JNKKTRmRuby2

   Plasmid

   #59148

   Purpose

   ORF encoding JNK KTR mRuby2 flanked by Gateway sequences

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   JNK Kinase Translocation Reporter (MAPK8 Mouse, Human)
   

   Tags

   mRuby2

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-HA-mRFP-pcDNA3.1-

   Plasmid

   #52510

   Purpose

   expresses mRFP in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   mRFP
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   engineered variant of tetrameric red fluorescent …

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSH231-Bx-GFP-C31

   Plasmid

   #115149

   Purpose

   Empty safe harbor site 231 knock-in vector with flanking homology arms and Bxb1 and C31 attP integrase landing pads

   Depositor

   Raymond Monnat, Michael Phelps

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   Safe Harbor Homology Sequences
   

   Expression

   Mammalian

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-lox-rox-STOP-rox-tRFP-pA-lox-ZsGreen-pA

    Plasmid

    #175438

    Purpose

    dual plasmid labelling cells of different neuronal population with different fluorophores

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

    Insert

    rox-STOP-rox-tRFP-pA
    

    Use

    Cre/Lox; Dre/rox

    Tags

    ZsGreen on backbone

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-p38KTRmCerulean3

    Plasmid

    #59149

    Purpose

    ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
    

    Available Since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMDC123SB-AtMIR390a-B/c

    Plasmid

    #51775

    Purpose

    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

    Depositor

    James Carrington

    Article

    Carbonell et al Plant Physiol. 2014 Mar 19.

    Insert

    AtMIR390a-B/c
    

    Use

    Plant expression

    Mutation

    A. thaliana MIR390a precursor sequence including …

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Axin2-luciferase

    Plasmid

    #21275

    Depositor

    Frank Costantini

    Article

    Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

    Insert

    Axin2 promoter (Axin2 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pGL3-KRAS

    Plasmid

    #13919

    Depositor

    Frank Slack

    Article

    Johnson et al Cell. 2005 Mar 11. 120(5):635-47.

    Insert

    KRAS 3'UTR (KRAS Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. Axin2-d2EGFP

    Plasmid

    #21276

    Depositor

    Frank Costantini

    Article

    Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

    Insert

    Axin2 promoter (Axin2 Mouse)
    

    Tags

    d2EGFP

    Expression

    Mammalian

    Available Since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-MT mouse Axin (Axin MTFu1)

    Plasmid

    #21287

    Depositor

    Frank Costantini

    Article

    Zeng et al Cell. 1997 Jul 11. 90(1):181-92.

    Insert

    Axin (Axin1 Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Dec. 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. CDK1as_T2A_Zeo

    Plasmid

    #118596

    Purpose

    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    CDK1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-3xCTCF-TetO

    Plasmid

    #213023

    Purpose

    piggyBac donor vector containing an 8-kb TetO operator array flanked by 3xCTCF sites for random integration using PBase transposase

    Depositor

    Luca Giorgetti

    Article

    Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

    Insert

    3xCTCF-TetO
    

    Use

    Mouse Targeting; Piggybase integration (transposo…

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPBC-G6s

    Plasmid

    #62812

    Purpose

    pPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-GCaMP6s-ITR
    

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEPQD0KN0750

    Plasmid

    #185627

    Purpose

    Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using AarI

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    lacZ
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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