We narrowed to 150,751 results for: ins

1. pTox-EGFPsite1_NGG-PAM (BPK740)

   Plasmid

   #181746

   Purpose

   p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGG PAM

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   ccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)
   

   Use

   Selection plasmid

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSP7-bio

   Plasmid

   #47735

   Purpose

   Expresses enzymatically monobiotinylated full-length MSP7 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised MSP7
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-GP-2-CBX7 Chromo

   Plasmid

   #59695

   Purpose

   Contains histone modification interacting domain (CBX7 Chromo)

   Depositor

   Albert Jeltsch

   Article

   Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

   Insert

   CBX7 Chromo (CBX7 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC8_GWs-Lox (VE5589)

   Plasmid

   #163769

   Purpose

   Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette under the pH promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   no insert
   

   Tags

   no tag

   Expression

   Insect

   Promoter

   PH

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMS3: pHelper(AvCAST)_AvPSP1_ΔTnsD

   Plasmid

   #168135

   Purpose

   Inducible expression of AvCAST proteins (except TnsD) with crRNA targeting AvPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array (with spacer for AvPSP1)
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. BTK-APEX2-P2A-mKate2

   Plasmid

   #67653

   Purpose

   Mammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   BTK-PH-APEX2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMS21: pHelper(PmcCAST)_entry

   Plasmid

   #168154

   Purpose

   Inducible expression of PmcCAST proteins. Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
   PmcTniQ
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSP5-bio

   Plasmid

   #47718

   Purpose

   Expresses enzymatically monobiotinylated full-length MSP5 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised MSP5
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. RhopH3-bio

   Plasmid

   #47781

   Purpose

   Expresses enzymatically monobiotinylated full-length RhopH3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised RhopH3
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. 1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

    Plasmid

    #149426

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Inserts

    U6.3-gRNAs[Sxl, bTub]
    Hsp70Bb-Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcrF2

    Plasmid

    #89234

    Purpose

    Plasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage site

    Depositor

    Blake Wiedenheft

    Article

    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

    Insert

    AcrF2
    

    Tags

    6xHis-TEV

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX Supt6h-SH2

    Plasmid

    #46518

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Supt6h (SUPT6H Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1317-1441

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. PAK7_HUMAN_D0

    Plasmid

    #79706

    Purpose

    This plasmid encodes the kinase domain of PAK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    PAK7 (PAK5 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-hSyn-LMO4 (GLucM23-VChR1-EYFP)

    Plasmid

    #114101

    Purpose

    fusion protein of Gaussia luciferase variant M23, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002

    Insert

    Gaussia luciferase, M23; Volvox Channelrhodopsin 1; EYFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MK13_HUMAN_D0

    Plasmid

    #79693

    Purpose

    This plasmid encodes the kinase domain of MK13. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    MK13
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pSG5-ERRbeta-delta10

    Plasmid

    #52187

    Purpose

    Expresses human ERRbeta-delta10 splice variant in mammalian cells

    Depositor

    Rebecca Riggins

    Article

    Heckler et al Cell Cycle. 2014 Dec 12:0.

    Insert

    Estrogen-related receptor beta (ESRRB Human)
    

    Expression

    Mammalian

    Mutation

    wild type sequence; codon optimized to exclude Ec…

    Promoter

    SV40

    Available Since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pGEX Nck-SH2

    Plasmid

    #46457

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Nck (NCK1 Human)
    

    Tags

    GST and Thrombin site

    Expression

    Bacterial

    Mutation

    contains amino acids 275-377

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX ShcA-SH2

    Plasmid

    #46486

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    ShcA (SHC1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 476-584

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMS6: pHelper(AvCAST)_entry_ΔTnsD

    Plasmid

    #168139

    Purpose

    Inducible expression of AvCAST proteins (except TnsD). Two BsaI sites for spacer cloning.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST minimal CRISPR array
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. mOrange2-Keratin-17

    Plasmid

    #57965

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Keratin (KRT18 Human)
    

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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