We narrowed to 8,454 results for: gnal

1. pMT-Bip-GFP:V5:KDEL

   Plasmid

   #69917

   Purpose

   An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

   Depositor

   Kenneth Irvine

   Article

   Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

   Insert

   GFP
   

   Tags

   Drosophila BiP signal sequence, KDEL ER targeting…

   Expression

   Insect

   Promoter

   pMT

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYHY3

   Plasmid

   #216109

   Purpose

   aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.

   Depositor

   Shannon Sirk

   Article

   Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

   Insert

   NanoLuc
   

   Use

   Synthetic Biology; E. coli-bacteroides shuttle ve…

   Tags

   3xFLAG tag and BT3630 signal peptide

   Expression

   Bacterial

   Promoter

   P1TDP-A21

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-3C-sGFP1-10_pcDNA3.1(-)

   Plasmid

   #182931

   Purpose

   For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

   Depositor

   William Weis

   Article

   Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

   Type

   Empty backbone

   Tags

   3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.23-MYC

   Plasmid

   #86461

   Purpose

   A modified version of the Promega pGL4.23 vector. Contains the MYC promoter in place of the minimal promoter and polyadenylation signals flanking the enhancer cloning site.

   Depositor

   Eric Lander

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   MYC

   Available Since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-Universal

   Plasmid

   #52694

   Purpose

   This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

   Depositor

   Sebastian Lourido

   Inserts

   Cas9
   Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
   

   Use

   CRISPR; Toxoplasma gondii

   Tags

   3X FLAG and Nuclear localization signal

   Promoter

   TgTUB1 and Toxoplasma U6

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAPV-DsRed2-ER-5

   Plasmid

   #170570

   Purpose

   Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   APV-DsRed2-ER-5
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ii-Str_SBP-mCherry-Ecadherin

   Plasmid

   #65289

   Purpose

   synchronize trafficking of Ecadherin from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ecadherin
   

   Tags

   IL-2 Signal Sequence and Streptavidin Binding Pro…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. dTomato_LD 0_TEV

   Plasmid

   #58872

   Purpose

   MESA protease chain with dTomato ectodomain and no linker domain

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA protease chain with dTomato ectodomain and TEV protease
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. GeNL(Ca2+)_480/pcDNA3

   Plasmid

   #85205

   Purpose

   luminescent reporter for calcium signaling

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   GeNL(Ca2+)_480
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Beta-Lactamase

    Plasmid

    #62729

    Purpose

    Expresses Beta-Lactamase protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    Beta-Lactamase
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Deletion of periplasmic signal domain

    Promoter

    T7

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHis17_ParM _1

    Plasmid

    #78201

    Purpose

    Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

    Depositor

    Martin Webb

    Article

    Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

    Insert

    parM (K33A I27C T174A T175N C287A)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    K33A I27C T174A T175N C287A

    Promoter

    unknown

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. ERoxBFP

    Plasmid

    #68126

    Purpose

    mammalian expression of ER localized oxBFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Insert

    oxBFP
    

    Tags

    KDEL ER retention sequence and bovine prolactin s…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

13. ER-moxMaple3

    Plasmid

    #120885

    Purpose

    mammalian expression of ER localized moxMaple3

    Depositor

    Erik Snapp

    Article

    Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

    Insert

    moxMaple3
    

    Tags

    KDEL ER retention sequence and bovine prolactin s…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ii-Str_SBP-EGFP-GPI

    Plasmid

    #65296

    Purpose

    synchronize trafficking of EGFP-GPI from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    GPI anchor
    

    Tags

    EGFP and IL-2 Signal Sequence and Streptavidin Bi…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJKR-H-cdaR

    Plasmid

    #62557

    Purpose

    Glucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Inserts

    beta lactamase
    sfGFP
    CdaR
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHEA

    Plasmid

    #168297

    Purpose

    Bacterial surface display of polypeptides (nanobodies) in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.

    Insert

    Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
    

    Tags

    6xHis, E-tag, and PelB signal peptide

    Expression

    Bacterial

    Promoter

    LacI-Plac

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-N1-jGCaMP7b-XN

    Plasmid

    #178362

    Purpose

    Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus.

    Depositor

    Xiaodong Liu

    Article

    Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

    Insert

    jGCaMP7b-XN
    

    Expression

    Mammalian

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV0765

    Plasmid

    #133501

    Purpose

    Expression of mCherry targeted to the nucleus with one NLS signals

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pLys3(BstZ17I)-p(tdh1)-NLSSV40-mCherry-terminator(ScADH1)-kanMX
    

    Expression

    Yeast

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAV0532

    Plasmid

    #133502

    Purpose

    Expression of mTagBFP2 targeted to the nucleus with one NLS signals

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pLys3(BstZ17I)-p(tdh1)-NLSSV40-mTagBFP2-terminator(tdh1)-kanMX
    

    Expression

    Yeast

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNM262

    Plasmid

    #128412

    Purpose

    Vanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    Lysostaphin (epr Staphylococcus simulans)
    

    Use

    Synthetic Biology

    Tags

    amyE SP

    Expression

    Bacterial

    Promoter

    Pspank(V)

    Available Since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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