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HA-DDX3

Plasmid

#44975

Depositor

Robin Reed

Article

Lee et al Nucleic Acids Res. 2008 Aug;36(14):4708-

Insert

DDX3 (DDX3X Human)

Tags

HA and Myc

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-R-iLACCO1.2

Plasmid

#208027

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1.2

Expression

Mammalian

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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PGK-H2BeGFP

Plasmid

#21210

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Phosphoglycerate kinase promoter (PGK1 Human)

Use

Lentiviral

Tags

H2B-eGFP

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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T-2A-EGFP-PGK-Puro

Plasmid

#83344

Purpose

Donor vector to knocin EGFP into human Brachyury(T) locus

Depositor

James Thomson

Article

Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

Insert

Human Brachyury knockin homology arms (TBXT Human)

Tags

2A-EGFP

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro_CMV_NES-Caprola_06-mEGFP

Plasmid

#194693

Purpose

CMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Use

Lentiviral

Tags

mEGFP

Promoter

CMV

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-hMALAT1

Plasmid

#118580

Purpose

to overexpress human MALAT1 in mammalian cells

Depositor

Jianjun Zhao

Article

Hu et al Leukemia. 2018 Mar 22. pii: 10.1038/s413

Insert

MALAT1 (MALAT1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

MSCV

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCCL/IFNB1-d2eGFP-3’UTR

Plasmid

#180232

Purpose

Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

Insert

d2eGFP

Use

Lentiviral

Promoter

1 kb upstream of IFNB1 CDS

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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mCh-Mfn2

Plasmid

#141156

Purpose

Mammalian expression and labeling mitofusin 2

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Mitofusin 2 (MFN2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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sUPRa

Plasmid

#223242

Purpose

sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels

Depositor

Colin Akerman

Article

Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10

Insert

sUPRa

Use

AAV

Promoter

Custom

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV BiP-Myc-KDEL-wt

Plasmid

#27164

Purpose

Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

Depositor

Ron Prywes

Article

Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

Insert

BiP (HSPA5 Human)

Tags

KDEL and Myc

Expression

Mammalian

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-Puro_siKD

Plasmid

#86695

Purpose

Tetracycline inducible expression of shRNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Expression

Mammalian

Promoter

H1 TO

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

Plasmid

#138151

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR_BsmBI

Use

Lentiviral

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc

Plasmid

#234826

Purpose

STITCHR R2Tocc editor

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

nCas9h840a-xten-R2Tocc

Use

CRISPR

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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T-STOP sgRNA-1

Plasmid

#83346

Purpose

The sgRNA at the stop codon of human Brachyury(T) locus

Depositor

James Thomson

Article

Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

Insert

T-STOP sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-eLACCO2.1-NGR

Plasmid

#208023

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Expression

Mammalian

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pTE_20 - pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-XTEN-BARD1-NLS-HbaUTR

Plasmid

#252016

Purpose

In vitro transcription of Cas9-BARD1 TruEditor for mRNA generation

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9-BARD1

Use

CRISPR

Expression

Mammalian

Mutation

NA

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_21 - pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-XTEN-BLM(1-193)-NLS-HbaUTR

Plasmid

#252017

Purpose

In vitro transcription of Cas9-BLM(2-194) TruEditor for mRNA generation

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9-BLM(2-194)

Use

CRISPR

Expression

Mammalian

Mutation

NA

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_22 - pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-XTEN-SLX1A-NLS-HbaUTR

Plasmid

#252018

Purpose

In vitro transcription of Cas9-SLX1A TruEditor for mRNA generation

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9-SLX1A

Use

CRISPR

Expression

Mammalian

Mutation

NA

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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CAG-FR-GECO1a

Plasmid

#163681

Purpose

Far-red fluorescent calcium indicator FR-GECO1a

Depositor

Robert Campbell

Article

Dalangin et al Nat Commun. 2025 Apr 7;16(1):3318. doi:

Insert

FR-GECO1a

Expression

Mammalian

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP

Plasmid

#215055

Purpose

Expresses EGFP in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

EGFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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