We narrowed to 19,015 results for: Met

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SRpHi4

Plasmid

#102544

Purpose

Bacterial expression of EYFP fused to Epidermal Growth Factor with a GST tag for purification

Depositor

Stefan Hell

Article

Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

Insert

EYFP-EGF

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCytERM-BDFP1.6-N1

Plasmid

#197179

Purpose

Expresses the fusion protein of CytERM-BDFP1.6 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-BDFP1.6

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDJSVT91

Plasmid

#109305

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tag

Depositor

Daniel Slade

Article

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Tags

SufI 1-30, 6His

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pOPTM p62 PB1 (1-102)

Plasmid

#210427

Purpose

Bacterial expression of MBP-PB1(1-102) with TEV cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 PB1 (1-102) (NUP62 Human)

Tags

MBP

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETb-tVYN-TmΔ

Plasmid

#149694

Purpose

High-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purification

Depositor

Ming Hammond

Article

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-TmYcgRΔ-NLucΔ4

Expression

Bacterial

Promoter

T7

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJKR-L-acuR

Plasmid

#62568

Purpose

Acrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pTK50_GFP-NuMA-N

Plasmid

#46348

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

NuMA N-terminal fragment (NUMA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

N-terminal fragment from 1-213

Promoter

pBABE

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pBp-Control Sponge (CXCR4 sponge)

Plasmid

#22744

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only
pJKR-L-cdaR

Plasmid

#62558

Purpose

Glucarate biosensor. Low copy plasmid that expresses GFP proportional to glucarate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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SRpHi2

Plasmid

#102542

Purpose

Bacterial expression of EGFP fused to the cell penetrating peptide TAT with a GST tag for purification

Depositor

Stefan Hell

Article

Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

Insert

Tat-EGFP

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pTK51_GFP-NuMA-C

Plasmid

#46349

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

NuMA C-terminal fragment (NUMA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

C-terminal fragment from 1700-2115

Promoter

pBABE

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pDH013

Plasmid

#157638

Purpose

sMHT-113 fused to GFP and RFP

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17-GFP
RFP-SYNZIP18-sMHT(114-23)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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SIRT5H158Y-pET15b

Plasmid

#81032

Purpose

Bacterial expression of Human catalytic mutant SIRT5 protein

Depositor

David Lombard

Article

Kumar et al Methods Mol Biol. 2016;1436:241-57. doi:

Insert

Sirt5 (SIRT5 Human)

Tags

His

Expression

Bacterial

Mutation

H158Y catalytic mutant

Promoter

T7

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLacZa-GFP

Plasmid

#86440

Purpose

visualization and quantifying the out-of-frame mutations from genome editing

Depositor

Yun-Bo Shi

Article

Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

Insert

LacZalpha-MCS-sfGFP fusion gene

Use

Color visualization in bacteria

Tags

LacZa-sfGFP

Promoter

Plac

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-DA

Plasmid

#184603

Purpose

For expression of a fluorescent sensor for dopamine in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-DA

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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mRuby-PH-PLCdelta

Plasmid

#162440

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

mRuby-PH-PLCdelta

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCytERM-miRFP680-N1

Plasmid

#197183

Purpose

Expresses the fusion protein of CytERM-miRFP680 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-miRFP680

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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mCh-FRB1C-Cb5

Plasmid

#162432

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

mCh-FRB1C-Cb5

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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SRpHi3

Plasmid

#102543

Purpose

Bacterial expression of sepHluorin fused to the cell penetrating peptide TAT with a GST tag for purification

Depositor

Stefan Hell

Article

Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

Insert

Tat-sepHluorin

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pKeratin-miRFP713-N1

Plasmid

#197235

Purpose

Expresses the fusion protein of Keratin-miRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Keratin-miRFP713

Expression

Mammalian

Available Since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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