We narrowed to 16,624 results for: puromycin

1. LentiCRISPR v2-sgLKB1 clone2

   Plasmid

   #162126

   Purpose

   Lentiviral sgRNA plasmid targeting human LKB1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgLKB1 (STK11 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-TetON-Puro-HIF1a 0819

   Plasmid

   #118704

   Purpose

   Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   shRNA against human HIF1a
   

   Use

   Lentiviral

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-eCas9

   Plasmid

   #140237

   Purpose

   Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   EF-1a core

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-TetON-Puro-HIF1a 3809

   Plasmid

   #118705

   Purpose

   Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   shRNA against human HIF1a
   

   Use

   Lentiviral

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-sh-mSOX9-5

   Plasmid

   #40646

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   sh-mSOX9-5 (Sox9 Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

   Plasmid

   #214875

   Purpose

   Empty lentiviral backbone for guide array cloning

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 7TFP CDH1 reporter

   Plasmid

   #91704

   Purpose

   lentiviral luciferase reporter containing CDH1 promoter

   Depositor

   Bob Weinberg

   Article

   Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

   Insert

   CDH1 promoter (CDH1 Human)
   

   Use

   Lentiviral and Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   CDH1

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSH-EFIRES-P-AtAFB2-mCherry

   Plasmid

   #129716

   Purpose

   AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   AFB2 (AFB2 Mustard Weed)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   EF1a

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMTL5

   Plasmid

   #127967

   Purpose

   DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLPCX mito Grx1-roGFP2

    Plasmid

    #64977

    Purpose

    Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    mitochondrial targeting sequence

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDG462

    Plasmid

    #100903

    Purpose

    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLPCX cyto Grx1-roGFP2

    Plasmid

    #64975

    Purpose

    Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pH2rU3_ForInd_mCherry_CMV_ZsGT2APurR

    Plasmid

    #204579

    Purpose

    lentivirus backbone cloning vector and template for barcoded non-neutralizable standard production

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    mCherry
    

    Use

    Lentiviral

    Promoter

    TRE3G

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRXIP-GFP-hATG2A

    Plasmid

    #114462

    Purpose

    Expresses human ATG2A with GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Tamura et al FEBS Lett. 2017 Dec;591(23):3819-3830. d

    Insert

    human ATG2A (ATG2A Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    P656R (please see depositors comment below)

    Promoter

    CMV

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXSIP-FLAG-ATPIF1

    Plasmid

    #204703

    Purpose

    expresses FLAG-ATPIF1 in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    ATP synthase inhibitory factor subunit 1 (ATP5IF1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9

    Plasmid

    #84242

    Purpose

    Expresses GA-inducible KRAB-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9
    GID1-KRAB
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAH1 (lenti hLPL-FLAG)

    Plasmid

    #102448

    Purpose

    FLAG-tagged human LPL in lentiviral vector

    Depositor

    Brandon Davies

    Article

    Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

    Insert

    Lipoprotein lipase (LPL Human)
    

    Use

    Lentiviral

    Tags

    6x His and FLAG

    Promoter

    CMV

    Available Since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBMN-HA-TBK1

    Plasmid

    #199205

    Purpose

    Used for the expression of TBK1 (SMC Internal No. 1996)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJM1 TMEM192-Flag

    Plasmid

    #112742

    Purpose

    express pLJM1 TMEM192-Flag

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    TMEM192-Flag (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAVS1-TLR targeting vector

    Plasmid

    #64215

    Purpose

    Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Insert

    traffic light reporter (TLR) (AAVS1 Human)
    

    Use

    Human targeting

    Mutation

    see publication for details

    Promoter

    CAG

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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