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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,426 results for: 11

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Showing: 6461 - 6480 of 7426 results

  3. pET15b I-SceI (Ctermhis) K223I

    Plasmid
    #195615
    Purpose
    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity
    Depositor
    Frederick Gimble
    Article
    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.
    Insert
    I-SceI
    Expression
    Bacterial
    Mutation
    C2694G, G2345C, G2351C, A3365T, A3366T
    Promoter
    T7
    Available Since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195615-plasmid-map-sequence-id-383680

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  4. pET15b I-SceI (Ctermhis) K122I

    Plasmid
    #195614
    Purpose
    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity
    Depositor
    Frederick Gimble
    Article
    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.
    Insert
    I-SceI
    Expression
    Bacterial
    Mutation
    C2458G, A2826T, A2827T
    Promoter
    T7
    Available Since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195614-plasmid-map-sequence-id-383676

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  7. hPCNA-S228I

    Plasmid
    #190938
    Purpose
    untagged human PCNA with a hypomorphic disease-associated serine to isoleucine mutation at residue 228
    Depositor
    Brian Kelch
    Article
    Duffy et al J Mol Biol. 2016 Mar 27;428(6):1023-1040
    Insert
    Proliferating Cell Nuclear Antigen (PCNA Human)
    Expression
    Bacterial
    Mutation
    changed serine 228 to isoleucine
    Promoter
    T7
    Available Since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    190938-plasmid-map-sequence-id-376466

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  10. OfCasp3a

    Plasmid
    #166053
    Purpose
    a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspase
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Orbicella faveolata caspase-3a
    Tags
    His tag
    Expression
    Bacterial
    Promoter
    T7 lac promoter
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166053-plasmid-map-sequence-id-328272

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  11. PaCasp7a

    Plasmid
    #166051
    Purpose
    a caspase of Porites astreoides, which has higher sequence similarity with human caspase-7 but has a CARD domain and function as an initiator caspase
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Porites astreiodes caspase-7a
    Tags
    His tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166051-plasmid-map-sequence-id-328269

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  12. AlkB-D135G

    Plasmid
    #160434
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Glycine; deleted the…
    Promoter
    T7
    Available Since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160434-plasmid-map-sequence-id-318954

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Showing: 6461 - 6480 of 7426 results