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pMuLE ENTR CMV/TO-miR-30 L5-L4

Plasmid

#62126

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV mCherry L5-L2

Plasmid

#62146

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and mCherry module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

mCherry

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV mCherry L1-R5

Plasmid

#62148

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and mCherry module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

mCherry

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221-ARL4

Plasmid

#67322

Purpose

hArl4 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Use

Gateway entry vector

Expression

Bacterial

Mutation

bp 105 A to T; silent mutation

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL4C

Plasmid

#67330

Purpose

hArl4c with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL2

Plasmid

#67318

Purpose

hArl2 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARF5

Plasmid

#67312

Purpose

hArf5 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Use

Gateway entry vector

Expression

Bacterial

Mutation

bp 477 T to C; silent mutation

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
Modified pYTK075

Plasmid

#254020

Purpose

A part plasmid designed to use the yeast selective marker (LEU2 gene) as a Level 1 TU fragment.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

LEU2

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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p028g13-MBP

Plasmid

#249631

Purpose

Clone MBP polypeptide in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3

Depositor

Isabel Abreu

Article

Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi

Insert

N-terminal tag with MBP polypeptide

Expression

Bacterial

Mutation

Silent mutation on Gly-20 to remove a BsaI recogn…

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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LentiGuideFUT8-neo

Plasmid

#250316

Purpose

Encodes gRNA targeting FUT8

Depositor

Jennifer Kohler

Article

Konada et al Glycobiology. 2026 Feb 26:cwag011. doi:

Insert

FUT8 gRNA (FUT8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/T119M

Plasmid

#242712

Purpose

bacterial expression of TTR

Depositor

Peter Wright

Article

Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, T119M

Promoter

T7

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/F87A

Plasmid

#238120

Purpose

bacterial expression of TTR

Depositor

Peter Wright

Article

Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, F87A

Promoter

T7

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR223-ABL2(WT)

Plasmid

#215467

Purpose

Gateway entry vector with Arg wild-type (WT)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ABL2 (ABL2 Human)

Use

Gateway entry vector

Mutation

Includes stop codon

Promoter

none

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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TTR A120S

Plasmid

#238111

Purpose

bacterial expression of human TTR mutant

Depositor

Peter Wright

Article

Sun et al Protein Sci. 2024 Sep;33(9):e5101. doi:

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

A120S

Promoter

T7

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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TTR A120L

Plasmid

#238110

Purpose

bacterial expression of human TTR mutant

Depositor

Peter Wright

Article

Sun et al Protein Sci. 2024 Sep;33(9):e5101. doi:

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

A120L

Promoter

T7

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR221-Illusia(YYEE)

Plasmid

#222591

Purpose

Gateway entry vector with a phospho-mimetic mutant (YYEE) of the Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(YYEE)

Use

Gateway entry vector

Mutation

Mutations in the NPxY sites equivolent to Y783E, …

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-Illusia(Y783F)

Plasmid

#222595

Purpose

Gateway entry vector with a phospho-mutant (Y783F) variant of the Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(Y783F)

Use

Gateway entry vector

Mutation

Mutation in the NPxY(783) site equivolent to Y783…

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-Illusia(Y795F)

Plasmid

#222597

Purpose

Gateway entry vector with a phospho-mutant (Y795F) variant of the Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(Y795F)

Use

Gateway entry vector

Mutation

Mutation in the NPxY(795) site equivolent to Y795…

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-Illusia(YYFF)

Plasmid

#215443

Purpose

Gateway entry vector with inactive phospho-mutant (YYFF) of Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(YYFF)

Use

Gateway entry vector

Mutation

Mutations in the NPxY sites equivolent to Y783F, …

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pB-DEST (JDW 1205)

Plasmid

#229821

Purpose

A PiggyBac destination vector compatible with gateway cloning system.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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