We narrowed to 9,277 results for: Pol;

1. pGEMHE-mClover3-bElys

   Plasmid

   #171494

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged bovine Elys mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   Elys (AHCTF1 B. tarus (bovine))
   

   Use

   Pgehme

   Tags

   mClover3

   Promoter

   T7

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX030

   Plasmid

   #167154

   Purpose

   MoClo-compatible Level 1 (position 5) vector encoding Neonothopanus nambi caffeoylpiruvate hydrolase nnCPH under control of 0.4 kb 35S promoter, for expression in plants

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   p35s-nnCPH-ocsT
   

   Expression

   Plant

   Promoter

   p35s

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAB120

   Plasmid

   #167150

   Purpose

   MoClo-compatible Level 1 (position 1) vector encoding Kanamycin resistance cassette, for expression in plants

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   pNOS-nptII-ocsT
   

   Expression

   Plant

   Promoter

   pNOS

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX022

   Plasmid

   #167148

   Purpose

   MoClo-compatible Level 0-CDS2 promoterless vector encoding C-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   C-part of fungal hispidin synthase, nnHispS
   

   Use

   Synthetic Biology

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX021

   Plasmid

   #167147

   Purpose

   MoClo-compatible Level 0-SP promoterless vector encoding N-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   N-part of fungal hispidin synthase, nnHispS
   

   Use

   Synthetic Biology

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)

   Plasmid

   #91806

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   Residues A1529-G1534 replaced with a PreScission …

   Promoter

   Endogenous Rpb1 promoter

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)

   Plasmid

   #91807

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   PreScission Protease site (LEVLFQGP) inserted bet…

   Promoter

   Endogenous Rpb1 promoter

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

   Plasmid

   #91818

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 1355 and lysine 1435 to alanines; …

   Promoter

   T7

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLJM1-Flag-Rheb-12Rs

   Plasmid

   #165023

   Purpose

   expression of Flag-Rheb-12Rs mutant protein in mammalian cells (all the lysine residues on Rheb except the K19 and K120 are mutated to arginines).

   Depositor

   Ken Inoki

   Article

   Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

   Insert

   Rheb-12Rs (RHEB Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   All the lysine residues except K19 and K120 are c…

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p1A Prk-

    Plasmid

    #162694

    Purpose

    p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Prk inactive (K20M S21A native protein, this map …

    Promoter

    PLtet0-1 promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330_gRNA_H19-DMR-2

    Plasmid

    #159930

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, CBh

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330_gRNA_H19-DMR-1

    Plasmid

    #159929

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, CBh

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-Mm_Plk5(300-595) PBD domain

    Plasmid

    #136316

    Purpose

    Mammalian expression of mouse Plk5-D300 (PBD domain 300-595) with N-terminal EGFP

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (Plk5 Mouse)
    

    Expression

    Mammalian

    Mutation

    fragment 300-595

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmirGLO-3'UTR-IL22

    Plasmid

    #153070

    Purpose

    Human IL-22 3'UTR region cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    3' UTR region of IL-22 gene (IL22 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDF11-SFPQ(214-598)_quadruple_mutant

    Plasmid

    #135441

    Purpose

    Expresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tag

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    splicing factor proline and glutamine rich (SFPQ Human)
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    L535A, L539A, L546A, M549A

    Promoter

    T7

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBacMam2-DiEx-LIC-N entry vector

    Plasmid

    #111751

    Purpose

    Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with N-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Type

    Empty backbone

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. PCRII apoBa

    Plasmid

    #121897

    Purpose

    Plasmid for making zebrafish apoBa in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoBa (apoba Zebrafish)
    

    Use

    Unspecified

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N1 Dynamin1 T78R L84R T92R V118R

    Plasmid

    #112106

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T78R L84R T92R V118R

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. hASXL(1-115)-luciferase

    Plasmid

    #106427

    Purpose

    Expresses human ASXL1 (amino acids 1-115) fused to the N-terminal of firefly luciferase

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    addition sex combs like 1 ASXL1 (ASXL1 Human)
    

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    amino acids 1-115 fused to luciferase

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJC20cdc8.27

    Plasmid

    #99362

    Purpose

    Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Expression

    Bacterial

    Mutation

    Glutamic acid 129 to Lysine

    Promoter

    T7

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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