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Omega Destination-CMV::ELuc:bGH

Plasmid

#124528

Purpose

Omega destination vector, that has a CMV:ELUC:bgH in the backbone

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA P1

Plasmid

#80943

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA P1 for Pd-l1 binding.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Ywhab-3'UTR

Plasmid

#61782

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhab

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhab 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Map3k1-3'UTR

Plasmid

#61776

Purpose

Luciferase reporter containing the 3'UTR of mouse Map3k1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Map3k1 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Fhod1-3'UTR

Plasmid

#61773

Purpose

Luciferase reporter containing the 3'UTR of mouse Fhod1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Fhod1 3'UTR

Use

Luciferase

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Mapk12-3'UTR

Plasmid

#61783

Purpose

Luciferase reporter containing the 3'UTR of mouse Mapk12

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Mapk12 3'UTR

Use

Luciferase

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
eSpCas9(1.1)_No_FLAG

Plasmid

#79877

Purpose

FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

Untagged eSpCas9(1.1)

Expression

Mammalian

Promoter

CBh

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pTK-RetE1-Citrine

Plasmid

#127779

Purpose

Enhancer reporter construct containing the chicken RetE1 enhancer driving Citrine activity

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

RetE1

Use

Enhancer reporter construct

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9-GCN4

Plasmid

#78899

Purpose

Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-10XGCN4

Use

CRISPR

Tags

HA

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pJC-20xUAS-TALE3-VP64-P10

Plasmid

#104607

Purpose

Expresses TALE3 under UAS control

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

TALE3

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJC-20xUAS-TALE4-VP64-P10

Plasmid

#104608

Purpose

Expresses TALE4 under UAS control

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

TALE4

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-CoChR-mRuby2-ST

Plasmid

#109131

Purpose

Cation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

CoChR-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLifeAct-miRFP713

Plasmid

#136562

Purpose

F-actin labeling with a near-infrared fluorescent protein miRFP713

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

LifeAct-miRFP713

Tags

miRFP713

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBPhsFlp2::PEST

Plasmid

#63171

Purpose

Drosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock Promoter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

hsFlp2::PEST

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pBBK

Plasmid

#62670

Purpose

Vector for PhiC31o mRNA synthesis

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

PhiC31

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pSV2 neuT

Plasmid

#10919

Depositor

Bob Weinberg

Article

Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

Insert

neuT (Erbb2 Rat)

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only
pBz15_Strep_Rubisco

Plasmid

#140839

Purpose

wild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

Form IA Rubisco; CbbL, CbbS

Tags

Strep II

Expression

Bacterial

Promoter

T7

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9

Plasmid

#78903

Purpose

Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B Flag BimEL

Plasmid

#23090

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pSV2 neuN

Plasmid

#10917

Depositor

Bob Weinberg

Article

Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

Insert

neuN (Erbb2 Rat)

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only