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pPD032 Reporter Template v1 (Mid Spaced)

Plasmid

#138753

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD033 Reporter Template v1 (Far Spaced)

Plasmid

#138754

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD529 VP16-ZF36

Plasmid

#138758

Purpose

Constitutive expression of VP16-ZF36 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF36

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE043 ZF43x1 mKate2 (TUPV1)

Plasmid

#138723

Purpose

mMoClo TUPV, with ZF43x1 promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x1

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x1

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE048 ZF43x6-S mKate2 (TUPV1)

Plasmid

#138728

Purpose

mMoClo TUPV, with ZF43x6-S promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Spaced

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x6-Spaced

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE047 ZF43x3-S mKate2 (TUPV1)

Plasmid

#138727

Purpose

mMoClo TUPV, with ZF43x3-S promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Spaced

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x3-Spaced

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE045 ZF43x6-C mKate2 (TUPV1)

Plasmid

#138725

Purpose

mMoClo TUPV, with ZF43x6-C promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x6-Compact

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE042 ZF43x0 mKate2 (TUPV1)

Plasmid

#138722

Purpose

mMoClo TUPV, with ZF43x0 promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x0

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x0

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE044 ZF43x3-C mKate2 (TUPV1)

Plasmid

#138724

Purpose

mMoClo TUPV, with ZF43x3-C promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x3-Compact

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE049 ZF43x12-S mKate2 (TUPV1)

Plasmid

#138729

Purpose

mMoClo TUPV, with ZF43x12-S promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x12-Spaced

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3b_019

Plasmid

#123800

Purpose

Encodes dsaCAS9 as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

SadCas9

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3_028

Plasmid

#124054

Purpose

Encodes c120 sg RNA guide cleaved by ribozyme

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RIbozyme (C120 sg RNA)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK8b_006

Plasmid

#123894

Purpose

Encodes the LoxP recognition site as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LoxP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK8b_008

Plasmid

#123895

Purpose

Encodes the 3' 500 bp cRosa26 locus homology arm as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LA_cROSA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK7_006

Plasmid

#123874

Purpose

Encodes the FRT cassette as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

FRT

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK6_006

Plasmid

#123860

Purpose

Encodes a spacer cassette as a type 6 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_006

Plasmid

#123820

Purpose

Encodes ER Targeting Sequence (N Terminal) as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

KDEL

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_008

Plasmid

#123822

Purpose

Encodes Golgi Targeting Sequence as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

GTS

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_012

Plasmid

#123825

Purpose

Encodes mScarlet as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK4a_015

Plasmid

#123828

Purpose

Encodes msfGFP as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

msfGFP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge