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D15_Ld_061240

Plasmid

#135831

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

thioredoxin, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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D13_Ld_050900

Plasmid

#135829

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

surface antigen- like protein

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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D12_Ld_040370

Plasmid

#135828

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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D11_Ld_211610

Plasmid

#135827

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
D6_Ld_100150

Plasmid

#135822

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

nucleoside phosphatase, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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D65_Ld_051210

Plasmid

#135878

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

surface antigen-like protein

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
D3_Ld_030930

Plasmid

#135819

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

multicopper oxidase, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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D1_Ld_290860

Plasmid

#135817

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

cysteine peptidase C (CPC)

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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D10_Ld_292250

Plasmid

#135826

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

C-8 sterol isomerase-like protein

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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D17_Ld_090870

Plasmid

#135833

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
D8_Ld_340400

Plasmid

#135824

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, unknown function

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pJM162

Plasmid

#134171

Purpose

Retroviral plasmid expressing GFP Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC 284-C 318AAA320 (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC 284-C 318AAA320

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a-T2-OgNLuc

Plasmid

#135951

Purpose

Expresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

OgNLuc LumiFluor

Tags

T2 mitochondrial localization sequence

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pIW093

Plasmid

#134137

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

CRISPR resistant, R155A

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pIW082

Plasmid

#134136

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

CRISPR resistant, R236A

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pIW088

Plasmid

#134135

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

CRISPR resistant, K223A K226A

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pIW085

Plasmid

#134134

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

CRISPR resistant, K183A K184A

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ150-x3.7

Plasmid

#124308

Purpose

Gateway entry vector with pcoCas9-x3.7 (Plant codon optimized)

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9-x3.7

Use

CRISPR

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ151D10A-x3.7

Plasmid

#124309

Purpose

Gateway entry vector with pcoCas9D10A-x3.7 (Plant codon optimized)

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9D10A-x3.7

Use

CRISPR

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pProEx-Htb-cytATL2-6Gly-sfGFP

Plasmid

#124065

Purpose

E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

cytATL-sfGFP

Tags

sfGFP

Expression

Bacterial

Promoter

trc

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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