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pNIC28-BSA4-G-S10

Plasmid

#127828

Purpose

Production of recombinant human ribosomal protein S10

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS10 (RPS10 Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 494-1290

Plasmid

#78580

Purpose

Expression flag-USP19 494-1290 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1290 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493 and mutated 1291 residue…

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pDEST myc MOV10 D645N

Plasmid

#51720

Purpose

plasmid expresses myc-tagged MOV10 D645N

Depositor

Markus Landthaler

Article

Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

Insert

MOV10 D645N (MOV10 Human)

Tags

myc

Expression

Mammalian

Mutation

D645N

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ERKsubDD-Venus-NES

Plasmid

#84630

Purpose

Encodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

ERKsubDD-Venus-NES

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2262A - [1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159851

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-GW1D1a(aa254-503)

Plasmid

#21520

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa254-503

Available Since

Sept. 21, 2009

Availability

Academic Institutions and Nonprofits only
pUWR-Cad-mTFP

Plasmid

#58327

Purpose

Destination vector for inserting ubi-Cad-mTFP to Drosophila melanogaster genome

Depositor

Denise Montell

Article

Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

Insert

Cad-mTFP

Expression

Insect

Promoter

poly-ubiquitin

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only
pZA27

Plasmid

#158505

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-4xMyc. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-4xMyc (ZAR1 Mustard Weed)

Tags

4xMyc

Expression

Plant

Mutation

L17E

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-GW1D5(aa1670-1962)

Plasmid

#21519

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa1670-1962

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pSKB3-SaGGR-I206F-L377H (JBEI-7202)

Plasmid

#110152

Purpose

pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-I206F-L377H mutant gene

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Tags

TEV-cleavable His-tag

Expression

Bacterial

Mutation

SaGGR-I206F-L377H mutant gene

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]

Plasmid

#159800

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2245 - [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159843

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-STAG2-S653Stop

Plasmid

#31976

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

changed Serine 653 to Stop codon

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
A102 ΔAmpH Δ2x+ve

Plasmid

#129484

Purpose

Expression of Glo3 ΔAmpH lacking the COPI-binding motif in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-GW1D10(aa566-1343)

Plasmid

#21517

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa566-1343

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pNH-TrxT-S15

Plasmid

#127822

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSAH0016

Plasmid

#79824

Purpose

Dual selection plasmid with lac operator, selection for binding with spectinomycin, for non-binding with chloramphenicol

Depositor

Katja Arndt

Article

Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

Inserts

cat
aadA

Promoter

AATTCAAAAAAATGCTTGACAATTAATCATCGGCTCGTATAATGTGTGG…

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-S15

Plasmid

#127823

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-160

Plasmid

#74607

Purpose

Expresses Hook domain of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-160 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pTW088

Plasmid

#115936

Purpose

3'UTR insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 3'UTR No E1

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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