We narrowed to 59,577 results for: ran

1. shRNA-Ratio-PosCon

   Plasmid

   #183553

   Purpose

   Designed to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio positive control (Pbrm1), Figure S2)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Pbrm1 shRNA
   

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. shRNA-Ratio-NegCon

   Plasmid

   #183554

   Purpose

   Designed to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio negative control (Luciferase), Fig S2)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Luciferase shRNA
   

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKDR-TetOn.13

   Plasmid

   #183584

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 13
   

   Expression

   Mammalian

   Promoter

   Tet-On

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKDR-EF1a.10

   Plasmid

   #183573

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 10
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKDR-EF1a.11

   Plasmid

   #183574

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 11
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKDR-EF1a.12

   Plasmid

   #183575

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 12
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKDR-EF1a.5

   Plasmid

   #183572

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 5
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKDR-EF1a.3

   Plasmid

   #183570

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 3
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKDR-EF1a.1

   Plasmid

   #183568

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 1
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKDR-CBh.21

    Plasmid

    #183565

    Purpose

    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

    Depositor

    Erik Dent

    Article

    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

    Insert

    Attenuator Sequence 21
    

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. psiCheck2-SV40p-3xFLAG-CMVp-mito-mTFP1

    Plasmid

    #182376

    Purpose

    Dual expression construct encoding 3xFLAG and mito-mTFP1 from separate promoters

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Inserts

    3xFlag
    mito-mTFP1
    

    Expression

    Mammalian

    Promoter

    CMV promoter and SV40 promoter

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEPQD0CM0058

    Plasmid

    #177022

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geraniol 8-oxidase; geraniol-10-hydroxylase; CYP76B6
    

    Use

    Synthetic Biology

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEPQD0CM0059

    Plasmid

    #177023

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    8-hydroxygeraniol oxidoreductase; 10-hydroxygeraniol oxidoreductase; alcohol dehydrogenase 10
    

    Use

    Synthetic Biology

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEPQD0CM0063

    Plasmid

    #177021

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geraniol synthase
    

    Use

    Synthetic Biology

    Mutation

    transit peptide removed

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJo55

    Plasmid

    #159623

    Purpose

    An empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.

    Depositor

    Vasiliy Taranov

    Article

    Lebedeva et al Biochemistry (Mosc). 2021 Sep;86(9):1128

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-HA-PGRMC1-1-54

    Plasmid

    #173472

    Purpose

    PGRMC1 lacking the C-terminal region downstream of the transmembrane domain

    Depositor

    Bill Tsai

    Article

    Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

    Insert

    PGRMC1 (PGRMC1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted for amino acids 55-194

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-HA-PGRMC1-21-195

    Plasmid

    #173473

    Purpose

    PGRMC1 lacking the N-terminal region upstream of the transmembrane region

    Depositor

    Bill Tsai

    Article

    Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

    Insert

    PGRMC1 (PGRMC1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted for amino acids 1-20

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAraQCas-BsaI

    Plasmid

    #170633

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
    

    Expression

    Bacterial

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSUMO chicken-cPLA2-C2-domain

    Plasmid

    #170753

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

    Insert

    PLA2G4A-C2 domain (PLA2G4A Chicken)
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJCC_084 Cas12i1 D647A

    Plasmid

    #171671

    Purpose

    For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1 D647A
    

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed aspartate 647 to alanine

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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