We narrowed to 10,563 results for: ESP

1. SARS-COV2-NSP1-HA-BASU

   Plasmid

   #154071

   Purpose

   Express SARS-CoV-2 NSP1 gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcsII-pb4-flag

   Plasmid

   #86768

   Purpose

   C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   proteasome beta subunit 4 (PSMB4 Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-WT-ATPIF1

   Plasmid

   #85403

   Purpose

   Retroviral vector expressing wild type human ATPIF1

   Depositor

   David Sabatini

   Article

   Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

   Insert

   ATPIF1 (ATP5IF1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Wildtype

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pCAG-mIRFP670nuc-TEV-Xph20-ETWV

   Plasmid

   #133044

   Purpose

   Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

   Insert

   Xph20-ETWV
   

   Tags

   NLS, TEV protease + TEV cleavage site, and miRFP6…

   Expression

   Mammalian

   Mutation

   S63K

   Promoter

   CAG

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJM1-PHD2

   Plasmid

   #223552

   Purpose

   Lentivirus transfer plasmid for expression of full length human PHD2

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

   Insert

   PHD2 (EGLN1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-MTS-KD-c-Src-FLAG

   Plasmid

   #44653

   Depositor

   Yoshimi Homma

   Article

   Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

   Insert

   v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
   

   Tags

   FLAG tag and mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   changed lysine 298 to methionine

   Promoter

   CMV promoter

   Available Since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-GG-OCT4-1-5-PGK-Puro

   Plasmid

   #102893

   Purpose

   PiggyBac transposon system construct with 5 concatenated U6 promoter driven transcriptional cassettes for the activation of OCT4. Contains PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   OCT4-gRNAs-1-5-PGK-puro (POU5F1 )
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac1_GI.1_N116C-G193C

   Plasmid

   #162580

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Expression

   Insect

   Mutation

   Asparagine 116 was mutated to Cysteine and Glycin…

   Promoter

   polyhedrin

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p426_Cas9_gRNA-ARS416d

   Plasmid

   #87386

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS416d sequence TAGTGCACTTACCCCACGTT in yeast chromosome 4.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS416d
   

   Use

   CRISPR

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEos3.2trCD45

    Plasmid

    #203753

    Purpose

    Encodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trCD45 (PTPRC Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAN19-3xFLAG-ccvTIR1-NOSt

    Plasmid

    #108546

    Purpose

    Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    3xFLAG

    Mutation

    Changed F79 to G

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCold I-Hero11

    Plasmid

    #187928

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero11

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero11 (C19orf53 Human)
    

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. px458_2A_GFP_sgRNA_EIF2AK2

    Plasmid

    #106108

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting EIF2AK2

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA targeting EIF2AK2 (EIF2AK2 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. CaMKIV-DCTK75E

    Plasmid

    #126423

    Purpose

    A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

    Depositor

    Douglas Black

    Article

    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

    Insert

    calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed lysine 75 to glutamic acid

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEos3.2FP

    Plasmid

    #203757

    Purpose

    Encodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    NRas c-term (NRAS Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.E2.ChETA

    Plasmid

    #220310

    Purpose

    Directs ChETA optogenetic actuator to fast spiking interneurons in cortex. Allows for single APs to be evoked.

    Depositor

    Matt Rowan

    Article

    Kumar et al Nat Commun. 2024 Apr 1;15(1):2823. doi:

    Insert

    ChETA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    S5E2

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-Rab27B GG

    Plasmid

    #89451

    Purpose

    Expression of GFP-tagged human Rab27B GG in mammalian cells

    Depositor

    Wendy Westbroek

    Article

    Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

    Insert

    human Rab27B GG (RAB27B Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    GG (geranyl geranyl site is mutated)

    Promoter

    CMV

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-CoV2-Omicron BA.1 spike

    Plasmid

    #194602

    Purpose

    Mammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)

    Depositor

    Ben Murrell

    Article

    Sheward et al Lancet Infect Dis. 2022 Jun;22(6):813-82

    Insert

    CoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac1_GI.1_Q62C-A140C

    Plasmid

    #162581

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    Glutamine 62 changed to Cysteine and Alanine 140 …

    Promoter

    polyhedring

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 Flag MFF iso2 S172A

    Plasmid

    #74392

    Purpose

    Flag tagged human S172A MFF isoform 2 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only