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Plasmid#112376PurposeCRISPR donor plasmid to tag human transcription factor ZNF169 with GFPDepositorInsertZNF169 homology arms flanking EGFP-IRES-Neo cassette (ZNF169 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZFP1-donor
Plasmid#112374PurposeCRISPR donor plasmid to tag human transcription factor ZFP1 with GFPDepositorInsertZFP1 homology arms flanking EGFP-IRES-Neo cassette (ZFP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF227-donor
Plasmid#112368PurposeCRISPR donor plasmid to tag human transcription factor ZNF227 with GFPDepositorInsertZNF227 homology arms flanking EGFP-IRES-Neo cassette (ZNF227 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTX1-donor
Plasmid#112364PurposeCRISPR donor plasmid to tag human transcription factor OTX1 with GFPDepositorInsertOTX1 homology arms flanking EGFP-IRES-Neo cassette (OTX1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF175-donor
Plasmid#112347PurposeCRISPR donor plasmid to tag human transcription factor ZNF175 with GFPDepositorInsertZNF175 homology arms flanking EGFP-IRES-Neo cassette (ZNF175 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF354C-donor
Plasmid#112339PurposeCRISPR donor plasmid to tag human transcription factor ZNF354C with GFPDepositorInsertZNF354C homology arms flanking EGFP-IRES-Neo cassette (ZNF354C Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF347-donor
Plasmid#112326PurposeCRISPR donor plasmid to tag human transcription factor ZNF347 with GFPDepositorInsertZNF347 homology arms flanking EGFP-IRES-Neo cassette (ZNF347 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-UCOE-dCas9-BFP-VP64
Plasmid#196717PurposeCRISPR-activation. dCas9-VP64 with TagBFP for sorting.DepositorInsertdCas9-TagBFP-VP64
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationD10A, N863APromoterEF1aAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-dCas9-VP64-BFP
Plasmid#196711PurposeCRISPR-activation. dCas9-VP64 with TagBFP for sortingDepositorInsertdCas9-VP64-T2A-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationD10A, N863APromoterEF1aAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpec5'
Plasmid#228549PurposeTemplate for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmidDepositorInsertTruncated 5' region of aadA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpec3'
Plasmid#228548PurposeTemplate for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmidDepositorInsertTruncated 3' region of aadA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB06
Plasmid#190480PurposeBase Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsCas9
XylR
UseE. coli / b. subtilis - c. difficile shuttle vect…TagsExpressionMutationPromoterxylR-driven promoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCUX1.1.0-gDNA
Plasmid#112434PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CUX1DepositorInsertCUX1 (CUX1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAZ.1.0-gDNA
Plasmid#112455PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MAZDepositorInsertMAZ (MAZ Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHRB.1.0-gDNA
Plasmid#112429PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor THRBDepositorInsertTHRB (THRB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQCi
Plasmid#154345PurposeSelf-Cutting and Integrating CRISPR backbone (px458 derivative with Cas9-intron, sgRNA, and bait integration sites)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-p300-Hygro
Plasmid#104411Purposetransient expression of dCas9-p300 fusion proteinDepositorInsertp300 (EP300 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVID
Plasmid#236635PurposeCRISPR cloning, gRNA expression, and detection of undesired vector insertion during HDR for DrosophilaDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterdU6:3Available sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pORFE1052
Plasmid#112078PurposeOverexpression of the AtCAS9 nickase in plant under 2x35S promoterDepositorInsertAtCAS9 D10A
UseCRISPRTagsExpressionPlantMutationPlant codon optimized nCas9 D10A S. pyogenesPromoter2x35SAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORFE0014
Plasmid#112076PurposeAtCAS9 nickase D10A module CDS1nsDepositorInsertAtCAS9 D10A without stop codon
UseCRISPRTagsExpressionPlantMutationPlant codon optimized dCas9 D10A S. pyogenesPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only