We narrowed to 4,282 results for: gem

1. pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5

   Plasmid

   #111818

   Purpose

   Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   Mef2c (Mef2c Mouse)
   Tdtomato
   Gata4 (Gata4 Mouse)
   Tbx5
   

   Use

   Cloning intermediate

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

   Plasmid

   #71707

   Purpose

   A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid

   Depositor

   Giulio Draetta

   Article

   Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16

   Insert

   humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
   

   Use

   CRISPR

   Tags

   3xFLAG and hGEM(1/110)

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSB-CMV-MCS-Puro GEM-LTB4

   Plasmid

   #202641

   Purpose

   Expresses the LTB4 sensor GEM-LTB4 in mammalian cells

   Depositor

   Balázs Enyedi

   Article

   Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

   Insert

   GEM-LTB4
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast

   Plasmid

   #197066

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBP

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   CRISPR

   Tags

   FKBP and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB-CMV-MCS-Puro GEM-LTB4mut

   Plasmid

   #202642

   Purpose

   Expresses the LTB4-insensitive control sensor GEM-LTB4mut in mammalian cells

   Depositor

   Balázs Enyedi

   Article

   Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

   Insert

   GEM-LTB4mut
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y)

   Plasmid

   #165489

   Purpose

   Xenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   2xLyn-ERex-Venus-bPAC(F198Y)
   

   Use

   Cdna expression, xenopus oocyte

   Tags

   Venus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

   Plasmid

   #119864

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

   Insert

   BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Tags

   HA-tagged biotin ligase (BirA), Thosea asigna vir…

   Expression

   Bacterial

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

   Plasmid

   #140200

   Purpose

   Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   dLbCas12a crRNA scaffold

   Promoter

   PgpdA

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

   Plasmid

   #140204

   Purpose

   Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Step 1 of cloning c…

   Promoter

   Aspergillus fumigatus U3 (RNAPIII).

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79889

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGB3_alpha2_BeYDV geminino 2.0 (no ATG)-eGFP (GB5178)

    Plasmid

    #225443

    Purpose

    Geminino 2.0-no ATG on CDS. BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1.

    Depositor

    Diego Orzaez

    Insert

    BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLXB3o1 HygR-MAR10-gemiGFP-MAR10 (GB4684)

    Plasmid

    #225439

    Purpose

    Module for stable transformation of Geminino 1.0-eGFP flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.

    Depositor

    Diego Orzaez

    Insert

    HygR-MAR10-gemiGFP-MAR10
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEMHE:mTREK-1 (S131C/C159A/C219A/C365A/C399A)

    Plasmid

    #224755

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A/C365A/C399A)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Mutation

    S131C/C159A/C219A/C365A/C399A

    Promoter

    T7

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEMHE:mTREK-1 (S131A/C159A/C219A/C365A/C399A)

    Plasmid

    #224756

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A/C365A/C399A)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Mutation

    S131A/C159A/C219A/C365A/C399A

    Promoter

    T7

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSII-EF-miRFP670v1-hGem(1/110)

    Plasmid

    #80006

    Purpose

    hGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    hGeminin (1/110) (GMNN Human)
    

    Use

    Lentiviral

    Tags

    miRFP670v1

    Promoter

    EF-1 alpha

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-mito-GEM-GECO1

    Plasmid

    #32461

    Purpose

    Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEM-GECO
    

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

    Promoter

    CMV

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEM-GFP-URA3-GFP

    Plasmid

    #72606

    Purpose

    Template for creating a C-terminal GFP tag AND an internal GFP tag from a single transfection of yeast

    Depositor

    Bruce Granger

    Article

    Granger et al PLoS One. 2018 Jan 12;13(1):e0191194. do

    Inserts

    GFP (full)
    URA3
    GFP (partial)
    Optional Linker
    

    Mutation

    Includes full 819 bp coding sequence of URA3, 435…

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEMT-Mef2c-tPT2A-GFP

    Plasmid

    #111771

    Purpose

    Bicistronic construct: Co-express two genes by 2A peptides

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Insert

    Mef2c-tPT2A-GFP (Mef2c Mouse)
    

    Use

    Cloning intermediate

    Tags

    GFP

    Promoter

    T7

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEM-3XP3.RFP-tan

    Plasmid

    #83474

    Purpose

    RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

    Depositor

    Patricia Wittkopp

    Article

    Lamb et al Fly (Austin). 2016 Aug 5:1-12.

    Insert

    3XP3.RFP-tan
    

    Use

    CRISPR

    Expression

    Bacterial and Insect

    Promoter

    3XP3 eye-expression Promoter

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEMHE-mCherry-mTrim21ΔRING-Box

    Plasmid

    #105523

    Purpose

    T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mCherry

    Mutation

    deletion of RING domain (aa124-462)

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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