Addgene

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pLC3RBSC2cI

Plasmid

#193775

Purpose

Expresses lambda CI under the control of PLLacO-1 promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

Lambda CI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLLacO-1

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pT7PI-Sce ESARC D326A

Plasmid

#195609

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D326A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Use

Tags

Expression

Bacterial

Mutation

T3593C, A3601C, T3602G

Promoter

T7

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pKC530 pBABE EGFP-ZNF335

Plasmid

#192698

Purpose

Retroviral transgene expression of human ZNF335

Depositor

Iain Cheeseman

Article

Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

Insert

ZNF335 (ZNF335 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCm No carrier NikJC199U

Plasmid

#174361

Purpose

Expression plasmid with a TEV removable C-term 8xHis Tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

Depositor

Brandon Greene

Article

Caceres et al Methods Enzymol. 2022;662:119-141. doi:

Insert

nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]

Use

Tags

8xHis and TEV

Expression

Bacterial

Mutation

C199U

Promoter

T7

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR 221-PHL2 (STOP)

Plasmid

#118683

Purpose

Gateway entry vector

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

PHL2 (AT3G24120 Mustard Weed)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR 221-XERICO (STOP)

Plasmid

#118685

Purpose

Gateway entry vector

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

XERICO (XERICO Mustard Weed)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST 527-PHL2

Plasmid

#118670

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

PHL2 (AT3G24120 Mustard Weed)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST 527-XERICO

Plasmid

#118671

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

XERICO (XERICO Mustard Weed)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST 544-PHL2

Plasmid

#118672

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

PHL2 (AT3G24120 Mustard Weed)

Use

Tags

His6-NusA

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST 544-XERICO

Plasmid

#118673

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

XERICO (XERICO Mustard Weed)

Use

Tags

His6-NusA

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST 565-PHL2

Plasmid

#118674

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

PHL2 (AT3G24120 Mustard Weed)

Use

Tags

His6-GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST 565-XERICO

Plasmid

#118675

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

XERICO (XERICO Mustard Weed)

Use

Tags

His6-GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST 566-PHL2

Plasmid

#118676

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

PHL2 (AT3G24120 Mustard Weed)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST 566-XERICO

Plasmid

#118677

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

XERICO (XERICO Mustard Weed)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBA cHA-FG1

Plasmid

#118665

Purpose

Plant overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

AT3G07200 (AT3G07200 Mustard Weed)

Use

Tags

HA

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBA cHA-XERICO

Plasmid

#118666

Purpose

Plant overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

XERICO (XERICO Mustard Weed)

Use

Tags

HA

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI743 dual-barcoded gRNA parent vector

Plasmid

#164915

Purpose

CEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid libraries

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI733 pRPR1(TetO)-RPC31-sgRNA

Plasmid

#164913

Purpose

For yeast genomic integration of sgRNA against RPC31

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

pRPR1(TetO)-RPC31-sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

RPR1

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI732 pRPR1(TetO)-HTS1-sgRNA

Plasmid

#164912

Purpose

For yeast genomic integration of sgRNA against HTS1

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

pRPR1(TetO)-HTS1-sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

RPR1

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCaggs-NLS-PAmCherry1-GSS-EGFP

Plasmid

#158000

Purpose

Expression of nucleus localized PAmCherry1 and EGFP fusion protein

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

NLS-PAmCherry1-GSS-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Actin promoter with CMV enhancer

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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