We narrowed to 18,457 results for: multi

1. pENTR-L3-SpCas9-HF2-2A-EGFP-L2

   Plasmid

   #141050

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   SpCas9-HF2-2A-EGFP
   

   Expression

   Mammalian

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-L3-Csy4-Cas9-L2

   Plasmid

   #141040

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Csy4-2A-Cas9
   

   Expression

   Mammalian

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR2-L3-SfoI-EGFP-PBL-R1

   Plasmid

   #141018

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   EGFP
   

   Expression

   Mammalian

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEU3-NII-HxHLICNot

   Plasmid

   #140183

   Purpose

   Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 12xHis-tag and NotI restriction site

   Depositor

   Tamás Mészáros

   Article

   Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

   Type

   Empty backbone

   Use

   Expression vector for a cell-free system

   Tags

   12xHis

   Promoter

   T7

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBz106_CsoS2_MR

   Plasmid

   #140843

   Purpose

   His-tagged CsoS2 Middle Region (MR), pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 Middle Region (MR)
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   only amino acids 257-603

   Promoter

   T7

   Available Since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLz75_CB_operon_CsoS2_N3-N4

   Plasmid

   #140857

   Purpose

   Same as pLz37 but with CsoS2 truncated such that NTD repeats N1 and N2 are removed

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delN1-N2), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-109 of CsoS2

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLz77_CB_operon_CsoS2_N3

   Plasmid

   #140859

   Purpose

   Same as pLz37 but with CsoS2 discontinuously truncated such that repeats N1, N2, and N4 are removed

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delN1-N2, N4), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-108 and 220-235 of CsoS2

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLz78_CB_operon_CsoS2_delNTD

   Plasmid

   #140860

   Purpose

   Same as pLz37 but with CsoS2 truncated to remove the entire NTD (i.e. N1, N2, N3, and N4 are removed)

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delNTD), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-235 of CsoS2

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLz76_CB_operon_CsoS2_N4

   Plasmid

   #140858

   Purpose

   Same as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2, and N3 are removed

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delN1-N3), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-189 of CsoS2

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBz109_CsoS2_NTD

    Plasmid

    #140842

    Purpose

    His-tagged CsoS2 N-terminal domain (NTD), pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 N-terminal domain (NTD)
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 262-869

    Promoter

    T7

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLz33_CsoS2_NTD_GFP

    Plasmid

    #140861

    Purpose

    His-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFP

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD fused to GFP
    

    Tags

    Hexahistidine tag and Superfolder GFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLz47_polyPro_CsoS2_Npeptide

    Plasmid

    #140845

    Purpose

    Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 N-peptide
    

    Tags

    Hexahistidine tag and Strep-tag II

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBz110_CsoS2_CTD

    Plasmid

    #140844

    Purpose

    His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 C-terminal domain (CTD)
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-594

    Promoter

    T7

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. p5E-161K enhancer

    Plasmid

    #138365

    Purpose

    Gateway compatible 5' entry clone contains human modified 161k enhancer sequence.

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    161k human enhancer
    

    Use

    Gateway compatible

    Mutation

    9 A bases deletion, that have no impact on the en…

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTK-EdnrbE2-Cerulean

    Plasmid

    #127771

    Purpose

    Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Cerulean activity

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    ednrbE2
    

    Use

    Enhancer reporter construct

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-Sox3gRNA-T2

    Plasmid

    #127778

    Purpose

    Chicken sox3 gRNA target site 2, cloned into the pU6 sgRNA (backbone #92359)

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    sox3gRNAT2
    

    Use

    CRISPR

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MTK4b_006

    Plasmid

    #123844

    Purpose

    Encodes spacer sequence (multicistronic) as a Type 4b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Spacer for multicistronic
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MTK4_006

    Plasmid

    #123814

    Purpose

    Encodes spacer sequence (multicistronic) as a Type 4 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Spacer for multicistronic
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQlinkH-YbATP-grasp

    Plasmid

    #129639

    Purpose

    Expression plasmid of ATP-grasp from Y. bercovieri

    Depositor

    Matthias Bochtler

    Article

    Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

    Insert

    YbATP-grasp
    

    Tags

    His6-tag

    Expression

    Bacterial

    Promoter

    pQE

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. S1_AscI_V5-gateway_Bsu36I

    Plasmid

    #128467

    Purpose

    destination vector with N-terminal V5 tag

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    destination vector with N-terminal V5 tag
    

    Expression

    Bacterial

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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