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pET15b-At 14-3-3 mu

Plasmid

#44938

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 mu (GRF9 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 phi

Plasmid

#44939

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 phi (GF14 PHI Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
EF2451_pET21a(+)

Plasmid

#13840

Depositor

SeWon Suh

Article

Kang et al Acta Crystallograph Sect F Struct Biol C

Insert

PPAT

Tags

His

Expression

Bacterial

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-Lin28b-P3

Plasmid

#64794

Purpose

Luciferase expression driven by Lin28b promoter P3

Depositor

Joshua Mendell

Article

Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Lin28b promoter P3 (LIN28B Human)

Use

Luciferase

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa

Plasmid

#233036

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

RfxCas13d-compatible gRNA expression cassette and hfCas13d

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Mesp1

Plasmid

#72687

Purpose

Doxycyline regulated expression of Mesp1 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Mesp 1 (MESP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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PB CRIV Gc Spike H6

Plasmid

#240163

Purpose

Plasmid for making PiggyBac stable cell line expressing Cristoli virus (CRIV) glycoprotein Gc spike with a C-terminal His6 tag

Depositor

Stephen Graham

Article

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

CRIV Gc spike

Use

Piggybac

Tags

6xHis

Expression

Mammalian

Mutation

contains residues 478-906 only

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pOPTH OROV N

Plasmid

#229663

Purpose

Express Oropouche virus BeAn 19991 nucleoprotein with N-terminal His6 tag

Depositor

Stephen Graham

Article

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

Nucleoprotein

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_hRNMT_RAM

Plasmid

#213528

Purpose

Expresses the human RNMT-RAM complex

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Inserts

human RNMT (RNMT Human)
RAM

Tags

His6-sumo

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_ZIKV_NS5_MTase

Plasmid

#213529

Purpose

Expresses the MTase domain of NS5 of ZIKV

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

ZIKA virus NS5 methyltransferase domain

Tags

His6-sumo

Expression

Bacterial

Mutation

MTase domain aa 1-265

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pINSPECT:NEAT1-total_SigP:NLuc

Plasmid

#197267

Purpose

Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

Insert

NEAT1-total homology arms

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEAHISSOarD1

Plasmid

#97067

Purpose

Expressing Sulfolobus solfataricus orf Sso Ard1 (N-terminal acetylase) protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus Sso Ard1 protein

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSIRV131

Plasmid

#97053

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 131 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 131

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSSO2462

Plasmid

#97064

Purpose

Expressing Sulfolobus solfataricus orf SSO2462 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO2462

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSSO2452

Plasmid

#97059

Purpose

Expressing Sulfolobus solfataricus orf SSO2452 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO2452

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF126

Plasmid

#97021

Purpose

Expressing Pyrobaculum spherical virus orf126 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 126

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPA5006

Plasmid

#97016

Purpose

Expressing Pseudomonas aeruginosa orf PA5006 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

P aeruginosa orf PA5006

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSIRV418/gp25

Plasmid

#97052

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 418/gp25 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 418/gp25

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDEHISSIRV119

Plasmid

#97054

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 119 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 119

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDEHISSIRV55

Plasmid

#97055

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 55 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 55

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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