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pCellFree_G03 VPS29

Plasmid

#67052

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

VPS29 (VPS29 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pFUS_A30A

Plasmid

#31029

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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Spt2-GFP

Plasmid

#115572

Purpose

Expresses yeast Spt2-GFP fusion protein

Depositor

Michael Downey

Article

Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

Insert

SPT2

Tags

GFP

Expression

Bacterial and Yeast

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pZH051

Plasmid

#47311

Purpose

Expresses Tsr-Venus-Ubiquitin-CI

Depositor

Jie Xiao

Article

Hensel et al Nat Struct Mol Biol. 2012 Aug;19(8):797-

Insert

wild type immunity region of lambda phage with tsr-venus-ub-cI

Tags

CI

Expression

Bacterial

Promoter

PR; PRM

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pJ291_WT_med

Plasmid

#128856

Purpose

Medium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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512 pME-miR

Plasmid

#26032

Depositor

Nathan Lawson

Article

Nicoli et al Nature. 2010 Apr 22. 464(7292):1196-200.

Insert

ef1a (eef1a1l1 Zebrafish)

Use

Entry vector

Available Since

Aug. 26, 2010

Availability

Academic Institutions and Nonprofits only
pHIE046 ZF43x12-C mKate2 (TUPV1)

Plasmid

#138726

Purpose

mMoClo TUPV, with ZF43x12-C promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x12-Compact

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 NFX1

Plasmid

#67131

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

NFX1 (NFX1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pSico Spry-2

Plasmid

#14809

Depositor

Tyler Jacks

Article

Shaw et al Genes Dev. 2007 Mar 15. 21(6):694-707.

Insert

Sprouty-2 shRNA (Spry2 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pPD355 VPR-FRB (NLS)

Plasmid

#138847

Purpose

Constitutive expression of VPR-FRB under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-FRB

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCellFree_G05

Plasmid

#67139

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis mCherry tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His and mCherry

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
cmv 16 E7 del PTLHE

Plasmid

#13688

Depositor

Karl Munger

Article

Gonzalez et al J Virol. 2001 Aug . 75(16):7583-91.

Insert

HPV 16 E7 del PTLHE (E7 Human)

Expression

Mammalian

Mutation

del aa 6-10

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pNLS-LSSmKate1-N1

Plasmid

#31870

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

CMV

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pcDWUER

Plasmid

#37094

Depositor

David Wang

Article

Gaynor et al PLoS Pathog. 2007 May 4. 3(5):e64.

Insert

WU polyomavirus early region (WUPyV_gp4, WUPyV_gp5 WU polyomavirus)

Expression

Mammalian

Promoter

CMV

Available Since

July 3, 2012

Availability

Academic Institutions and Nonprofits only

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pSARSF505

Plasmid

#46832

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

GFPN-NpuDnaE_intein-GFP_C

Tags

GFP_C and H6-GFP_N

Expression

Bacterial

Mutation

stemmer GFP with superfolder mutations S30R and Y…

Promoter

T7

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-TetR-Rad18UBD

Plasmid

#119010

Purpose

Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

TetR

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV0-OCT-HA-eGFP

Plasmid

#67479

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 S100A1

Plasmid

#67048

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

S100A1 (S100A1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G06

Plasmid

#67140

Purpose

Gateway destination vector for cell-free expression with C-term mCherry 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

HIS and mCherry

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pTagRFP675-actin-C1

Plasmid

#44277

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

Insert

TagRFP675

Tags

Actin

Expression

Mammalian

Mutation

M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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