Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,837 results for: pET

Clear keywords
Showing: 6601 - 6620 of 9837 results

  1. rpoDs366C

    Plasmid
    #129688
    Purpose
    Expression of Sigma 70 S366C
    Depositor
    Jeff Gelles
    Article
    Mumm et al bioRxiv 2020
    Insert
    rpoD (rpoD E. coli)
    Tags
    His6/thrombin
    Expression
    Bacterial
    Mutation
    C132S C291S C295S S366C
    Available Since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    129688-plasmid-map-sequence-id-270569

    Enlarge

  3. pJB02

    Plasmid
    #111959
    Purpose
    Expresses GST-GFP-DHFR; construct generated for pull-down and localization experiments
    Depositor
    Matthew Good
    Article
    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259
    Inserts
    mEGFP
    eDHFR
    Tags
    DHFR
    Expression
    Bacterial
    Mutation
    eDHFR;residues 2-159 and enhanced GFP with monome…
    Promoter
    T7
    Available Since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111959-plasmid-map-sequence-id-219913

    Enlarge

  4. 4YWQ

    Plasmid
    #69953
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RC3H1:YTC019-B07:C232791 (RC3H1 Human)
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. mRuby-CD151-7

    Plasmid
    #55850
    Purpose
    Localization: Tetraspanin/Membrane, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    CD151 (CD151 Human)
    Tags
    mRuby
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. 2P0E

    Plasmid
    #60109
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    C9orf95:C9orf95_dml-X01:C201158 (NMRK1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. KIF13B

    Plasmid
    #25309
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KIF13B:S4-K351:cH6 (KIF13B Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids S4-K351
    Available Since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. pmSod1-G93A-N’His

    Plasmid
    #117705
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    G93A
    Promoter
    T7
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117705-plasmid-map-sequence-id-227526

    Enlarge

  11. pKK-mTagBFP-TEV

    Plasmid
    #105772
    Purpose
    Expression of your protein of interest in fusion with blue fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mTagBFP-TEV
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105772-plasmid-map-sequence-id-203361

    Enlarge

  12. pJK234

    Plasmid
    #73324
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis proteins VioA, VioB, and VioC
    Depositor
    T. J. Kappock
    Inserts
    violacein biosynthesis protein VioA
    violacein biosynthesis protein VioB
    violacein biosynthesis protein VioC
    Expression
    Bacterial
    Promoter
    T7 and n/a
    Available Since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    73324-plasmid-map-sequence-id-135032

    Enlarge

  13. NEDD4L-C2

    Plasmid
    #25615
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NEDD4L-C2 (NEDD4L Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains C2 domain
    Available Since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. pKD072

    Plasmid
    #136472
    Purpose
    SSB-C-term-GFP fusion expression plasmid
    Depositor
    James Keck
    Article
    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60
    Insert
    SSB-C-term-GFP (ssb E. coli)
    Tags
    superfolder GFP
    Expression
    Bacterial
    Mutation
    superfolder GFP attached to C-terminus at Phe 178
    Promoter
    T7 promoter
    Available Since
    April 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    136472-plasmid-map-sequence-id-267962

    Enlarge

  18. 4QQD

    Plasmid
    #61947
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UHRF1:SDC130-H02:C39674 (UHRF1 Human)
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. ARHGAP11AA

    Plasmid
    #25261
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ARHGAP11AA:M1-L253 (ARHGAP11A Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids M1-L253
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pHybr_r2a>mG2a-srt-his

    Plasmid
    #124803
    Purpose
    HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2a
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124803-plasmid-map-sequence-id-244318

    Enlarge

Showing: 6601 - 6620 of 9837 results