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p49.unipolyA.Cdt1.mSca.mCMV.PGK.eGFP.Gem1.WPRE

Plasmid

#226768

Purpose

vector with mScarlet fluorescent protein and GFP

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Type

Empty backbone

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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apt-CBE-SL3

Plasmid

#219940

Purpose

Mammalian expression of apt-CBE-SL3 protein (MCP_16aa-linker_evoAPOBEC1) with P2A-EGFP marker

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

MCP_16-aa_evo-rA1_2xUGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-BASU-His

Plasmid

#218451

Purpose

E coli expression vector for Halo-BASU-His biotin ligase

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

HaloTag-BASU-His fusion protein

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRDB_268

Plasmid

#231112

Purpose

SpG-Cas9

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

Cas9-SpG

Use

CRISPR and Lentiviral

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA516

Plasmid

#231118

Purpose

Fragmid fragment: (2A Selection) CBE activity-based selection intron

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

PuroN'-intron-PuroC'

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA566

Plasmid

#231115

Purpose

Fragmid fragment: (guide cassette) ABE activity-based selection cloning

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA568

Plasmid

#231117

Purpose

Fragmid fragment: (guide cassette) CBE activity-based selection cloning

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA509

Plasmid

#231116

Purpose

Fragmid fragment: (2A Selection) ABE activity-based selection intron

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

PuroN'-intron-PuroC'

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTrc-clbS_Frischella

Plasmid

#223358

Purpose

Encoding ClbS from Frischella perrara

Depositor

Emily Balskus

Article

Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

Insert

Encoding ClbS from Frischella perrara (Codon optimized for E coli)

Expression

Bacterial

Promoter

pTrc

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pTrc-clbS_Mixta

Plasmid

#223352

Purpose

Encoding ClbS from Mixta theicola

Depositor

Emily Balskus

Article

Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

Insert

Encoding ClbS from Mixta theicola (Codon optimized for E coli)

Expression

Bacterial

Promoter

pTrc

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pTrc-clbS

Plasmid

#223350

Purpose

Encoding wild type ClbS from E. coli CFT073

Depositor

Emily Balskus

Article

Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

Insert

ClbS from CFT073 (Codon optimized for E coli)

Expression

Bacterial

Promoter

pTrc

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAMC070n1a12

Plasmid

#209746

Purpose

ColA BBa_B0014 tetAp SD8 ScTrpRS rrnB1 proB SD8 Lum1PylRS BBaB10014RNAItrpA+ J23100 SD8 MmPylRS T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

tRNA operon - ScTrpRS - Lum1PylRS - MmPylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKDC.118

Plasmid

#227188

Purpose

T7 IPTG-driven Cas13a

Depositor

Seth Shipman

Article

Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

Insert

LbuCas13a

Expression

Bacterial

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB170

Plasmid

#218591

Purpose

Express engineered TFs Adr1c(S230A), Pip2c, and Oaf1c

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Adr1

Expression

Yeast

Mutation

Adr1(S230A), Pip2(1-168 + 2,497-2,988), Oaf1(1-30…

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1340

Plasmid

#218593

Purpose

Express a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22. Also overexpresses Pex11

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex22

Expression

Yeast

Mutation

Pex22(1-36)

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSKT001

Plasmid

#218594

Purpose

Express degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Tags

UbiY degradation tag and ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1357

Plasmid

#218595

Purpose

Express Pex11 and the degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex11

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pKS1461

Plasmid

#218587

Purpose

Express peroxisomal Erg12p, Erg8p, and Mvd1p to convert mevalonate to IPP

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Erg12

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa50H9

Plasmid

#179628

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa50H9

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa3G5

Plasmid

#179629

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa3G5

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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