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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,348 results for: multi

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Showing: 6661 - 6680 of 18348 results

  1. pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

    Plasmid
    #51256
    Purpose
    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu
    Depositor
    Rajat Rohatgi
    Publication
    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gli2 (Gli2 Mouse)
    Use
    Flpin system
    Tags
    3xHA-TEV
    Expression
    Mammalian
    Mutation
    amino acids 216,220,224,230,248 mutated to Glu
    Promoter
    EF1a
    Available since
    Feb. 25, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag

    Plasmid
    #216696
    Purpose
    Synchronize trafficking of TNFalpha from the ER (RUCH system)
    Depositor
    Franck Perez
    Publication
    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    FIREmate-KDEL
    Tumor Necrosis Factor alpha (TNFa)
    Tags
    FIREtag, Streptavidin Binding Protein (SBP), and …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216696-plasmid-map-sequence-id-429707

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  7. PB-PEmax SpG

    Plasmid
    #187895
    Purpose
    All-in-one prime editor piggyBac transposon with PEmax, SpG variant
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    BPSV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    187895-plasmid-map-sequence-id-370756

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  8. pcDNA3-Lyn-dPlcR(K30/32L)

    Plasmid
    #181833
    Purpose
    Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.
    Depositor
    Jin Zhang
    Publication
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-dPlcR (K30L,K32L)
    Tags
    N-terminal targeting sequence from Lyn kinase, dd…
    Expression
    Mammalian
    Mutation
    Lysines 30 and 32 in PH domain mutated to leucine.
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181833-plasmid-map-sequence-id-364220

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  9. pcDNA3-Lyn-dInPAkt(R23/25A)

    Plasmid
    #181835
    Purpose
    Phospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.
    Depositor
    Jin Zhang
    Publication
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-dInPAkt
    Tags
    N-terminal targeting sequence from Lyn kinase, dd…
    Expression
    Mammalian
    Mutation
    Arginines 23 and 25 in PH domain mutated to alani…
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181835-plasmid-map-sequence-id-364313

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  10. pStr-KDEL_TPV-CXCL9-mCherry-SBP

    Plasmid
    #170677
    Purpose
    Expresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal TPV tripeptide (cargo) for RUSH.
    Depositor
    Ke Xu
    Publication
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CXCL9 (CXCL9 Synthetic, Human)
    streptavidin-KDEL
    Tags
    SBP and mCherry
    Expression
    Mammalian
    Promoter
    IRES and T7
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170677-plasmid-map-sequence-id-335797

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  11. pCRISPaint-mScarlet-BlastR [M1G]

    Plasmid
    #171814
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Publication
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mScarlet-T2A-BlastR
    Use
    Gene tagging
    Mutation
    BlastR: Changed Methionin 1 to Glycine.
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    171814-plasmid-map-sequence-id-338118

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  12. pACT1:Cas9-GFP, U6:sgUPRT

    Plasmid
    #122853
    Purpose
    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene
    Depositor
    David Sibley
    Publication
    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9-GFP
    U6-sgUPRT
    Use
    CRISPR; Cas9-gfp plasmid for genome editing of cr…
    Tags
    eGFP
    Promoter
    Cryptosporidium parvum U6 and Cryptosporidium par…
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    122853-plasmid-map-sequence-id-238877

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  13. eGFP-PRM-5R

    Plasmid
    #112155
    Purpose
    Mammalian expression plasmid for PRM-5R in a vector expressing eGFP.
    Depositor
    Michael Rosen
    Publication
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PRM-5R (ABL1 Human)
    Expression
    Mammalian
    Mutation
    contains only the PRM domain of Abl1
    Promoter
    CMV
    Available since
    Feb. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    112155-plasmid-map-sequence-id-236127

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  16. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

    Plasmid
    #87827
    Purpose
    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Publication
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    NLS
    PTS1
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87827-plasmid-map-sequence-id-175008

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  17. pPonA-BI-Gl NORM-LacZA TER- LacZB

    Plasmid
    #86212
    Purpose
    beta Globin NMD construct with and without PTC under Bi-directional Ponasterone A inducible promoter
    Depositor
    Robert Singer
    Publication
    Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    beta-Globin (HBB, Hbb Mouse, Human)
    Use
    Ponasterone a inducible promoter
    Tags
    lacZA or lacZB
    Expression
    Mammalian
    Mutation
    39TER in beta Globin in MCS II
    Promoter
    Ponasterone A inducible
    Available since
    Jan. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    86212-plasmid-map-sequence-id-169958

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  19. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

    Plasmid
    #194885
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-1 and -2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Publication
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_WT
    U6-crRNA1and2
    Use
    Lentiviral
    Tags
    HA
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    194885-plasmid-map-sequence-id-382817

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  20. pMOS012E: Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

    Plasmid
    #163074
    Purpose
    Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)
    Depositor
    Adam Cohen
    Publication
    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Grx1-roGFP1-iEER
    Use
    Gateway Cloning
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    163074-plasmid-map-sequence-id-325106

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Showing: 6661 - 6680 of 18348 results