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We narrowed to 14,067 results for: LEA

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Showing: 6681 - 6700 of 14067 results

  1. Ce iPGM-C-HiBiT

    Plasmid
    #207120
    Purpose
    Bacterially expressed iPGM-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Publication
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    C. elegans-iPGM HiBIT His10 (ipgm-1 Nematode)
    Tags
    HiBiT Tag and Thrombin cleavage site + 10x His Ta…
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    207120-plasmid-map-sequence-id-417169

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  2. T7 Lig-C-HiBiT

    Plasmid
    #215441
    Purpose
    Bacterially expressed T7 Lig-C-HiBit for Ni-NTA purification
    Depositor
    James Inglese
    Publication
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Escherichia phage T7 DNA Ligase, C-terminal HiBiT, C-terminal 10XHis.Thrombin tag
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215441-plasmid-map-sequence-id-428723

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  4. pEJS2696-CMV-Nme2SmuCas9-D56R

    Plasmid
    #228717
    Purpose
    Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nme2SmuCas9-D56R nuclease
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D56R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228717-plasmid-map-sequence-id-454003

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  7. t206-405

    Plasmid
    #166132
    Purpose
    For expression of human talin-1 head fragment (residues 206-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Publication
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    T1head1-F2F3(206-405) (TLN1 Human)
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 206-405 only
    Available since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166132-plasmid-map-sequence-id-330268

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  8. pGBW-m4046878

    Plasmid
    #145743
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145743-plasmid-map-sequence-id-298347

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  9. pGBW-m4046839

    Plasmid
    #145646
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145646-plasmid-map-sequence-id-323990

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  10. pGBW-m4046770

    Plasmid
    #145594
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 leader protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145594-plasmid-map-sequence-id-323992

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  11. pGBW-m4046797

    Plasmid
    #145676
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 leader protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 27, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145676-plasmid-map-sequence-id-292488

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  13. pEvolvR-mCherry-NNG-Slug-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249079
    Purpose
    Expresses nucleus-localized NNG-Slug-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    NNG-Slug-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249079-plasmid-map-sequence-id-496312

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  15. pTol1-U6abc_cmlc2(myl7)_cmlc2-nmKate

    Plasmid
    #238374
    Purpose
    Drives expression of 3 different gRNAs targeting cmlc2 (myl7), and expression of nuclear mKate in cardiomyocytes.
    Depositor
    Juan Manuel González-Rosa
    Publication
    Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nuclear mKate/3 gRNAs targeting cmlc2
    Promoter
    cmlc2 (nmKate); U6 (gRNAs)
    Available since
    Nov. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    238374-plasmid-map-sequence-id-480246

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  16. TVMV-TEV-HRV-3C Proteases-mCherry

    Plasmid
    #243798
    Purpose
    Encodes for mammalian expression of TVMV, TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
    Depositor
    Cole DeForest
    Publication
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TVMV, TEV and HRV-3C proteases
    Expression
    Mammalian
    Available since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243798-plasmid-map-sequence-id-486451

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  17. p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2

    Plasmid
    #227775
    Purpose
    Expression of beta actin driven BFP with multiple cloning site at 3' end. Also contains heat-shock activated mCherry reporter and CreERT2.
    Depositor
    Bushra Raj
    Publication
    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    BFP-MCS
    mCherry-t2A-CreERT2
    Use
    Zebrafish expression
    Promoter
    Olactb and hsp70l
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    227775-plasmid-map-sequence-id-455547

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  18. SpRY

    Plasmid
    #198544
    Purpose
    Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpRY
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198544-plasmid-map-sequence-id-390296

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  19. SpG

    Plasmid
    #198543
    Purpose
    Expresses human codon-optimized SpG and blasticidin resistance: EFS promoter-SpG-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpG
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    198543-plasmid-map-sequence-id-388887

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  20. pETSUMO2_Vitiosangium-bGSDM-thrombin

    Plasmid
    #222099
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Publication
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Vitiosangium-bGSDM-thrombin ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222099-plasmid-map-sequence-id-437094

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Showing: 6681 - 6700 of 14067 results