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Plasmid#44160DepositorInsertTak1 (Map3k7 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/TO-hEPO
Plasmid#50437Purposeexpress human erythropoietin under the control of tetracycline-regulatable CMV promoter using lentiviral delivery systemDepositorInsertEPO (EPO Human)
UseLentiviral; Tetracycline-regulatable (t-rex)TagsExpressionMammalianMutationPromoterCMV/TOAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
EF.delmFasL.CMV.GFP
Plasmid#17620DepositorInsertdelFasL (Fasl Mouse)
UseLentiviralTagsExpressionMammalianMutationNoncleavable mouse delFasL . Deleted the exon 2 r…PromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
KAT14-pDEST10
Plasmid#118382PurposeHIS-tagged insect cell expression vector with KAT14.DepositorInsertKAT14 (KAT14 Human)
UseTagsHISExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Coff/FonEYFP
Plasmid#55652PurposeCre-off/Flp-on EYFP under the Synapsin promoterDepositorInserteYFP with introns
UseAAV; Cre off/flp on eyfpTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mScarlet
Plasmid#169219PurposeTargeting vector backbone to support a knock-in of Linker-mScarlet at the C-terminus of a target locusDepositorInsertDouble SapI flanked mScarlet
UseTargeting vector backboneTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
TVBB N-term-mScarlet
Plasmid#169218PurposeTargeting vector backbone to support a knock-in of mScarlet-Linker at the N-terminus of a target locusDepositorInsertDouble SapI flanked mScarlet
UseTargeting vector backboneTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-3xFLAG-YAP1-S127A
Plasmid#42239DepositorInsertYAP1 (YAP1 Human)
UseGatewayTags3xFLAGExpressionMutationSerine 127 changed to alaninePromoternoneAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pELPS-eDHFR-YFP-T2A-Renilla
Plasmid#193253PurposeTriple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferaseDepositorInsertThe eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
UseLentiviralTagsRenilla luciferase (rLuc) and yellow fluorescent …ExpressionMutationNonePromoterEF1aAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-dL5**-mCER-TRF1
Plasmid#168176PurposeExpresses FAP (dL5**) fused to mCerulean and the telomere binding protein TRF1DepositorInsertTRF1 (TERF1 Human)
UseLentiviralTagsFAP (dL5**) and mCeruleanExpressionMammalianMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_DIO_mMeCP2-HaloTag
Plasmid#164062PurposeDouble-Floxed Inverted Open reading frame for mouseMeCP2(alpha) Under EF1a promoterDepositorInsertMeCP2 Isoform 1 (Mecp2 Mouse)
UseAAVTagsHaloTagExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc1/bC
Plasmid#74049Purposeretroviral expression plasmid for human NFATc1/bCDepositorInserthuman NFATc1, isoform beta-C (NFATC1 Human)
UseRetroviralTagsno tagExpressionMammalianMutationPromoterpMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-BLM DM
Plasmid#80071PurposeExpression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutationsDepositorInsertBLM (BLM Human)
UseTagsGFP-BLMExpressionMammalianMutationK317R (AAA>AGA) and K331R (AAA>AGA)PromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-U1AF37MF77M
Plasmid#168267PurposeExpresses human dmU1A with the F37M/F77M double mutantDepositorInsertU1A RRM1 crystallization module (SNRPA Human)
UseTags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F37M and F77MPromoterT7 promotorAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2-TetR-neoR
Plasmid#153310PurposeTet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GWDepositorInsertTetR
UseTransposonTagsExpressionMutationPromoterSV40Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER-21-RUNX1-P2A-ERG
Plasmid#97045PurposeExpresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.DepositorInsertRunt Related Transcription Factor 1, ETS-Related Gene
UseLentiviralTagsExpressionMammalianMutationPromoterTet onAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RR
Plasmid#108860PurposeLbCpf1 Gateway entry plasmid with G548R and K611R mutationsDepositorInsertLbCpf1-RR (LbCpf1 with G548R and K611R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationG548R and K611R, Cpf1 is rice codon optimizedPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Con/Foff EYFP
Plasmid#55651PurposeCre-on/Flp-off EYFP under the Synapsin promoterDepositorInserteYFP with introns
UseAAV; Cre on/flp off eyfpTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSP2440
Plasmid#72250PurposeHuman expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…PromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIG-LRRC37Bchimpanzee-HA
Plasmid#220794PurposeTo express the chimpanzee receptor LRRC37B in mammalian cells together with EGFPDepositorInsertLRRC37B
UseTagsHAExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only