We narrowed to 15,981 results for: LEA

1. pcDNAintron-CHIKV-Structural polyprotein with Nluc tagged E2

   Plasmid

   #215699

   Purpose

   Produces the Chikungunya virus structural polyprotein Capsid-E3-NLucE2-6K-E1

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV structural polyprotein Capsid-E3-NLucE2-6K-E1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAAV-EF1a-ArgiNLS-miRFP670

   Plasmid

   #220600

   Purpose

   Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   miRFP670
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)

   Plasmid

   #220597

   Purpose

   Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   mVenus-Q69M (ME)
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-ArgiNLS-mKate2

   Plasmid

   #220599

   Purpose

   Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   mKate2
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-FLEX-ArgiNLS-EGFP

   Plasmid

   #220601

   Purpose

   Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   ArgiNLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOTTC1706 - pAAV mGrid1 390F gRNA EF1a EGFP-KASH

   Plasmid

   #131683

   Purpose

   An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a guide RNA for mGrid1

   Depositor

   Christopher Richie

   Article

   Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

   Inserts

   EGFP-KASH
   SpCas9 sgRNA vs mouse GRID1
   

   Use

   AAV

   Tags

   KASH

   Promoter

   EF1a and mU6

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

   Plasmid

   #195018

   Purpose

   An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

   Depositor

   Christopher Richie

   Article

   Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

   Inserts

   CCGTATAGGCGGCTGCCCAA
   EGFP-KASH
   TTCCGTGGTGGGCAACGTAG
   

   Use

   AAV

   Tags

   KASH domain of Nesprin2

   Promoter

   EF1a, hU6, and mU6

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSAVED-CHAT

   Plasmid

   #214041

   Purpose

   Bacterial expression plasmid for SAVED-CHAT from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. KU70-eGFP-SUN1ΔN

   Plasmid

   #213516

   Purpose

   Expresses human KU70-eGFP-SUN1ΔN in mammalian cells

   Depositor

   Karim Mekhail

   Article

   Shokrollahi et al bioRxiv 2023.05.07.539750

   Insert

   KU70-eGFP-SUNΔN
   

   Expression

   Mammalian

   Mutation

   SUN1ΔN has the first 138 amino acid (lamina domai…

   Promoter

   CMV

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. DRD2-V2tail-TevN-BLITz-TetR-FKBP

    Plasmid

    #210514

    Purpose

    expresses DRD2-V2tail-TevN-BLITz-TetR-FKBP component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    DRD2-V2tail-TevN-BLITz-TetR-FKBP
    

    Tags

    HA signal FALG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. WT_IFL_G1A-T2A-A28C_R

    Plasmid

    #200185

    Purpose

    WT PA14 I-F Leader and G1A-T2A-A28C Repeat

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)
    

    Expression

    Bacterial

    Mutation

    G1A-T2A-A28C in the I-F repeat

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-DIO-GRAB_g5-HT2h

    Plasmid

    #208715

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT2h in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT2h
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFSynW SYT9 D197, 199N IRES GFP

    Plasmid

    #195701

    Purpose

    Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Mutation

    full-length mouse SYT9 with D197, 199N mutations

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFSynW SYT9 D330, 332N IRES GFP

    Plasmid

    #195702

    Purpose

    Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Mutation

    full-length mouse SYT9 with D330, 332N mutations

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. PET-HypaR-SpCas9-NLS-6xHis

    Plasmid

    #207387

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HypaR-SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R661A, N692A, M694A, Q695A, H698A

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PET-SpCas9-HF1-NLS-6xHis

    Plasmid

    #207382

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. B-HiFi SpCas9

    Plasmid

    #207362

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HiFi SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R691A, amino acids 1005-1013 replaced with two gl…

    Promoter

    CBh

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBactin-AcGFP-CI

    Plasmid

    #196849

    Purpose

    Expression of green fluorescent reporter AcGFP driven by the strong Beta-actin promoter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    AcGFP
    

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. PET-HeFSpCas9-NLS-6xHis

    Plasmid

    #207391

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HeFSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PET-B-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207378

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A, amino acids 1005-1013 replaced with two gl…

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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