We narrowed to 31,224 results for: LEA

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pISFba1

Plasmid

#226820

Purpose

Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISFba1 guide RNA targeting pMB1 ori

Depositor

Sheng Yang

Article

Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

Insert

ISFba1

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pISFba1reRNA

Plasmid

#226822

Purpose

Expressing ISFba1 guide RNA targeting maeB site controled by PJ23119

Depositor

Sheng Yang

Article

Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

Insert

ISFba1 none target guide RNA

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pOCC154

Plasmid

#118902

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette

Tags

TwinStrep, cleavable with 3C protease

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET26b-Granzyme A (S-A)

Plasmid

#8825

Depositor

Judy Lieberman

Article

Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

Insert

Human Granzyme A inactive (GZMA Human)

Tags

(His)6

Expression

Bacterial

Mutation

changed Serine 184 to Alanine

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only
Caspase-6

Plasmid

#100102

Purpose

Bacterial expression of Caspase-6

Depositor

Jim Wells

Article

Seaman et al Cell Death Differ. 2016 Oct;23(10):1717-

Insert

Caspase-6 (CASP6 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pOCC7

Plasmid

#118876

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette

Tags

HIS6, cleavable with 3C protease

Expression

Insect

Promoter

polH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-eA3AmaxNG-BlastR

Plasmid

#152999

Purpose

C-to-T base editor, NG PAM

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-nSpCas9 NG-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pOCC153

Plasmid

#118901

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette

Tags

TwinStrep, cleavable with 3C protease

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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YTIP-GFP

Plasmid

#42238

Depositor

William Pu

Article

von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

YTIP-GFP

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV miRNA control plasmid

Plasmid

#31508

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Type

Empty backbone

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
p2T-CMV-eA3Amax-T31A-NG-BlastR

Plasmid

#153000

Purpose

C-to-T/G/A base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-BE4 T31A-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF-L3-NF-L1-dMb2Cas12a

Plasmid

#177686

Purpose

Gateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

2xFokI-dMb2Cas12a

Use

CRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…

Tags

NLS

Expression

Plant

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-ERK2 T183AY185F

Plasmid

#118299

Purpose

Expression of Rat ERK2

Depositor

Philip Stork

Article

Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

Insert

ERK2 T183AY185F (Mapk1 Rat)

Tags

GFP

Expression

Mammalian

Mutation

T183AY185F

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRG645_lexO-AscI_LexA-TF_LEU2MX

Plasmid

#154813

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-ninein coiled-coil 2

Plasmid

#73522

Purpose

Encodes the second coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein coiled-coil 2 (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-ninein coiled-coil 1

Plasmid

#73521

Purpose

Encodes the first coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein coiled-coil 1 (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only
pRG714_lexO-AscI_LexA-TF_URA3MX

Plasmid

#154822

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-flag-mKdm4d(H189A)-polyA

Plasmid

#61554

Purpose

For mRNA synthesis or expression in mammalian cells

Depositor

Yi Zhang

Article

Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

Insert

Kdm4d (catalytic mutant)

Tags

flag

Expression

Mammalian

Mutation

changed Histidine 189 (of Kdm4d) to Alanine

Promoter

CMV and T7

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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3xEGFP-mMeikin

Plasmid

#174708

Purpose

Mammalian expression of mouse Meikin

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin (Meikin Mouse)

Tags

3xEGFP

Expression

Mammalian

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTYF-sGFAP-IRES-tdTomato

Plasmid

#166920

Purpose

Empty plasmid backbone; includes IRES-tdTomato and sGFAP promoter

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Type

Empty backbone

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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