We narrowed to 60,228 results for: PLE

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pcDNA5FRT/Flag-hINO80N2 (1-406)

Plasmid

#29442

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 1-406 (a.a.)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
ArpC3-FLAG_pLib

Plasmid

#173680

Purpose

ArpC3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC3 (ARPC3 Human)

Tags

TEV cleavage site and FLAG tag

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-hINO80N1 (1-262)

Plasmid

#29441

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 1-262 (a.a.)

Available Since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only
pEMS1920

Plasmid

#82562

Purpose

High copy number plasmid containing Ple253 (PITX3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

Ple253

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1941

Plasmid

#82564

Purpose

High copy number plasmid containing Ple264 (NR2E1 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

Ple264

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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MZB035

Plasmid

#217834

Purpose

Bacterial expression of human AP3D1 (1-617) and AP3S1 AP-3 core hemicomplex

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Inserts

AP3D1 (AP3D1 Human)
AP3S1 (AP3S1 Human)

Tags

GST

Expression

Bacterial

Mutation

Removed residues 618-1153 from AP3D1

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLIC-His6-MBP-TEV-Rgs14

Plasmid

#236395

Purpose

Expresses His6-MBP-TEV-Rgs14 in E. coli

Depositor

John Hepler

Article

Brown et al J Biol Chem. 2015 Apr 3;290(14):9037-49.

Insert

Rattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)

Tags

hexahistidine (H6) tag and maltose-binding protei…

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

Plasmid

#199390

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Tags

14xHis-Avi-SUMOEu

Expression

Bacterial

Promoter

T5-LacO

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

Plasmid

#113862

Purpose

Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CMV-eGFP
TOP-NLuc1.1
12GLI-Fluc
CBF-GLuc

Use

Lentiviral

Expression

Mammalian

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pIVM_26

Plasmid

#204501

Purpose

Expression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)

Depositor

Alessio Ciulli

Article

Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

Insert

EloB (1-104) and EloC (17-112) (ELOC, ELOB Human)

Expression

Bacterial

Mutation

0

Promoter

AmpR

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE-BI-osTIR1

Plasmid

#207840

Purpose

Inducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

Depositor

Michaela Mueller-McNicoll

Article

Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

Inserts

osTIR
mCherry
mAID_-VHH-GFP4

Tags

3X HA-Tag and weak NLS

Expression

Mammalian

Mutation

All K mutated to R

Promoter

TRE3G BI promoter

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac1-His10FLAG

Plasmid

#157736

Purpose

empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

Depositor

Debra Hansen

Article

Olson et al Protein Expr Purif. 2021 May 7:105890. d

Type

Empty backbone

Tags

His10 + 3xFLAG, enterokinase-cleavable

Expression

Insect

Promoter

polyhedrin

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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2xMARS

Plasmid

#205233

Purpose

Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cells

Depositor

Jeremy Baskin

Article

Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816

Insert

two repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)

Tags

Nuclear Export Sequence and mScarlet-i

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-His10TEV

Plasmid

#159427

Purpose

empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

Depositor

Debra Hansen

Article

Olson et al Protein Expr Purif. 2021 May 7:105890. d

Type

Empty backbone

Tags

TEV-protease-cleavable His10-tag

Expression

Insect

Promoter

polyhedrin

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE-BI-TRIM21

Plasmid

#207838

Purpose

Inducible TRIM21 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

Depositor

Michaela Mueller-McNicoll

Article

Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

Inserts

TRIM21 (TRIM21 Human)
mCherry
hIgG1-FC
VHH-GFP4

Tags

weak NLS

Expression

Mammalian

Mutation

All K mutated to R

Promoter

TRE3G BI promoter

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

Plasmid

#217512

Purpose

KD Recombinase dependent conditional smGdP-10XOllas cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

Tags

smGdP-10XOllas

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-GFP(1-10)opti

Plasmid

#129416

Purpose

Expressing codon-optimized GFP(1-10) fragment in human cells

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

codon-optimized GFP(1-10)

Use

CRISPR, TALEN, and Unspecified ; Human safe harbo…

Expression

Mammalian

Promoter

EF-1α

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pFIN-RK-GFP-WPRE

Plasmid

#44358

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

RK-GFP (MAPK14 Synthetic, Human)

Use

Lentiviral

Promoter

human rhodopsin kinase (pos 2557-2853)

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA

Plasmid

#217511

Purpose

KD Recombinase dependent conditional smGdP-10XHA cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA

Tags

smGdP-10XHA

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE-BI-aaAFB2

Plasmid

#207839

Purpose

Inducible AFB2 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

Depositor

Michaela Mueller-McNicoll

Article

Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

Inserts

atAFB2
mCherry
miniIAA7
VHH-GFP4

Tags

3X FLAG-Tag and weak NLS

Expression

Mammalian

Mutation

All K mutated to R

Promoter

TRE3G BI promoter

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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