We narrowed to 33,219 results for: PLE
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Plasmid#27380DepositorInsertCep290 (Cep290 Mouse)
TagsmCherryExpressionMammalianMutationplease see depositor comments belowAvailable SinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFC
Plasmid#214950PurposeBacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation.DepositorInsertsVNp-anti-Lysozyme Fab heavy chain amino-BiFC fusion
VNp-anti-Lysozyme Fab light chain amino-BiFC fusion
Tags6xHis, Amino half of venus BIFC fragment, Carboxy…ExpressionBacterialPromoterT7Available SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
GPR171-DuET
Plasmid#213269PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-ATG13 (2-197)
Plasmid#188075PurposeTo make ATG101/ATG13 HORMA complex for expression in mammalian cellsDepositorInsertATG13 (ATG13 Human)
Tagsstrep-strep-Flag tagExpressionMammalianMutationATG13 (2-197)PromoterCMVAvailable SinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL1 CTFdeltaTA-FLAG
Plasmid#217739PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL1 (ADGRL1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-844Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR149
Plasmid#106049PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR149; Opto-GPR149; E3)DepositorInsertOpto-GPR149 (GPR149 Human, Bovine)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR39
Plasmid#106021PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR39; Opto-GPR39; B11)DepositorInsertOpto-GPR39 (GPR39 Human, Bovine)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas12a-ABE8e-V106W
Plasmid#193646PurposeExpression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-TadA*8e-V106W, EGFP
ExpressionMammalianMutationTadA*8e-V106WPromoterCMVAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR84-DuET
Plasmid#213292PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas12a-ABE7
Plasmid#193644PurposeExpression of base editor dCas12a-ABE7 in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-TadA-TadA*7.10, EGFP
ExpressionMammalianPromoterCMVAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dCas9-3xNLS-VP64 (Construct 1)
Plasmid#55195PurposeExpresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.DepositorInsertdCas9
UseCRISPR and Synthetic BiologyTagsVP64ExpressionMammalianPromoterCMV/hUBCAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
Gene Trap - 24XMS2 Lentiviral Vector
Plasmid#174198PurposeLentiviral expression vector coding for Blasticidin, iRFP and 24X MS2 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.DepositorInsertiRFP
UseLentiviralTags24X MS2 hairpins, Blasticidin, and T2AAvailable SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRB2 CTFdeltaTA-FLAG
Plasmid#217715PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRB2 (BAI2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-917Available SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL4 CTFdeltaTA-FLAG
Plasmid#217742PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL4 (ADGRL4 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-412Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL3 CTFdeltaTA-FLAG
Plasmid#217741PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL3 (ADGRL3 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-847Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRB3 CTFdeltaTA-FLAG
Plasmid#217716PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRB3 (BAI3 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-862Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG1 CTFdeltaTA-FLAG
Plasmid#217732PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG1 (ADGRG1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-388Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRB1 CTFdeltaTA-FLAG
Plasmid#217714PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRB1 (ADGRB1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-932Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF5 CTFdeltaTA-FLAG
Plasmid#217731PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF5 (ADGRF5 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-996Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG5 CTFdeltaTA-FLAG
Plasmid#217736PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG5 (ADGRG5 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-232Available SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only