We narrowed to 10,030 results for: Pol
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Plasmid#111568PurposeExpresses a plasma membrane calcium pump hPMCA2w/bDepositorHas ServiceAAV5InsertPlasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
UseAAVTagsmCherryAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mScarlet-hCenpC
Plasmid#171490PurposeT7 promotor drives in vitro transcription of mScarlet-tagged human hCenpC mRNADepositorAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRE-Lepr_FL-IRES-eGFP
Plasmid#196635PurposeDoxycycline inducible expression of full length mouse Lepr with eGFP markerDepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_WT USP7
Plasmid#131242PurposeMammalian expression of N-terminally Myc-tagged USP7DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianPromoterCMV enhancer + CMV promoterAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-LaminA
Plasmid#231894PurposeExpression of HaloTag-tagged LaminA to visualize the nuclear laminaDepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
Pdg459-eSp(1.1) V3
Plasmid#226965PurposeCBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Cep170 (Nigg PID200)
Plasmid#41150DepositorAvailable SinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo_BACE1
Plasmid#227105PurposeBACE1 enzyme overexpression for Nrg1 activation in luciferase and barcode assaysDepositorInsertBACE1 (BACE1 Human)
ExpressionMammalianAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,Δ42a]
Plasmid#49332PurposeRepaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
ExpressionMammalianMutationContains exons 8a, 11, and 31b.Exons 32 and 42a a…PromoterCMVAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-ART
Plasmid#174810PurposeBacterial expression of isolated PARP1 ART subdomainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-BRCT
Plasmid#174807PurposeBacterial expression of isolated PARP1 BRCT domainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-WGR
Plasmid#174808PurposeBacterial expression of isolated PARP1 WGR domainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]
Plasmid#196883PurposeDual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporterDepositorInsertroxSTOProx-ZsGreen-bGH
UseCre/Lox; Dre/roxExpressionMammalianPromoterQuimeric CAGAvailable SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-mTurquoise-MLC-IRES-neo
Plasmid#85145Purposelentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoiseDepositorInsertmTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)
UseLentiviralTagsmTurquoisePromoterEF1aAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAP03-sp44/p21
Plasmid#120232PurposeCarries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.DepositorInsertbidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
UseSynthetic BiologyMutationaac(3)IV (apramycin resistance gene)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pB80-hsKIF5B(1-353)-GCN4-L-mCherry
Plasmid#193715PurposeExpression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353)DepositorInserthsKIF5B(1-353)-GCN4-L-mCherry (KIF5B Synthetic, Human)
TagsmCherryExpressionMammalianMutationhsKIF5B(aa1-353); mCherry: Met1 DeletedPromoterChicken beta-actinAvailable SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB2
Plasmid#63103PurposeExpression of HSPB2 (small HSP) tagged with V5 in mammalian cellsDepositorAvailable SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYLL201
Plasmid#53454PurposeExpresses HA-tagged mouse DNA topoisomerase IIbeta (full length cDNA, in pCS2+ backbone)InsertHA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) (Top2b Mouse)
TagsHAExpressionMammalianPromoterCMVAvailable SinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)
Plasmid#208977PurposeEntry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)DepositorInsertpUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
UseCRISPRTagsBPNLSExpressionMammalianPromoterhuman U6Available SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIB-HIS-TEV-CKS1
Plasmid#177013PurposeGenerate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEVDepositorInsertCKS1 (CKS1B Synthetic, Human)
TagsPolyhistidine-TEVExpressionInsectMutationcodon-optimised for insect cell expressionAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only