We narrowed to 9,186 results for: mel

1. pEANHISECArdB

   Plasmid

   #96967

   Purpose

   Expression of E. coli ArdB protein wirh an N-terminal TEV protease cleavable His tag

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   E. coli ArdB
   

   Tags

   N-ter TEV cleavable 6His

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1α-FLEX-rc[Chronos-GFP]

   Plasmid

   #62725

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   April 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR

   Plasmid

   #157744

   Purpose

   knock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expression

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   chlorotoxin containing chimeric antigen receptor targeting glioblastoma
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TGFbetaR1 (T204D)-pcw107-V5

   Plasmid

   #64629

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   TGFBR1 (transcript variant 1) (TGFBR1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   T204D

   Promoter

   PGK

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

5. INCTbiosyn-pEF-GFP(rc)Bxb1

   Plasmid

   #127511

   Purpose

   Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT/T0-Flag-RECQL5

   Plasmid

   #222620

   Purpose

   Mammalian expression vector of flag-tagged RECQL5

   Depositor

   Daniel Durocher

   Article

   Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

   Insert

   RECQL5 (RECQL5 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. INCTbiosyn-pEF-GFP(rc)phiC31

   Plasmid

   #127510

   Purpose

   Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-FLEX-rc [Chronos-tdTomato]

   Plasmid

   #84484

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe.puro.CDK8.flag

   Plasmid

   #19758

   Depositor

   William Hahn

   Article

   Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

   Insert

   cyclin-dependent kinase 8 (CDK8 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Dec. 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3TS-zSpG

    Plasmid

    #179316

    Purpose

    In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Zebrafish codon-optimized Cas9 variant SpG
    

    Use

    CRISPR; In vitro transcription by t3 promoter

    Tags

    SV40-NLS

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT3TS-zSpRY

    Plasmid

    #179317

    Purpose

    In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Zebrafish codon-optimized Cas9 variant SpRY
    

    Use

    CRISPR; In vitro transcription by t3 promoter

    Tags

    SV40-NLS

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28b-SpG-His

    Plasmid

    #179318

    Purpose

    Plasmid for bacterial expression and purification of SpG-Cas9

    Depositor

    Julián Cerón, Miguel Angel Moreno-Mateos

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Human codon-optimized Cas9 variant SpG
    

    Use

    CRISPR

    Tags

    6xHis and SV40-NLS

    Expression

    Bacterial

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Notch3 intracellular domain-pcw107-V5

    Plasmid

    #64623

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    NOTCH3 (NOTCH3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    intracellular domain *

    Promoter

    PGK

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pDONR223-ZNF423

    Plasmid

    #88083

    Purpose

    Donor Vector containing ZNF423 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF423 (ZNF423 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe.puro.CDK8.KD.flag

    Plasmid

    #19759

    Depositor

    William Hahn

    Article

    Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

    Insert

    cyclin-dependent kinase 8 (CDK8 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D173A (kinase dead)

    Available Since

    Dec. 11, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-Syn-FLEX-rc[Chronos-GFP]

    Plasmid

    #62722

    Purpose

    AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    human synapsin promoter

    Available Since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-FRT/T0-Flag-BRIP1

    Plasmid

    #222619

    Purpose

    Mammalian expression vector of flag-tagged BRIP1

    Depositor

    Daniel Durocher

    Article

    Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

    Insert

    BRIP1 (BRIP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pDECKO_GFP

    Plasmid

    #141210

    Purpose

    empty GFP vector for cloning sgRNA or pgRNAs

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Syn-FLEX-rc[CoChR-GFP]

    Plasmid

    #62724

    Purpose

    AAV-mediated expression of CoChR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    CoChR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    human synapsin promoter

    Available Since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1/BCR-FGFR1

    Plasmid

    #167490

    Purpose

    Mammalian expression vector for the BCR-FGFR1 fusion gene associated with myeloid and lymphoid neoplasms

    Depositor

    Nicholas Cross

    Article

    Demiroglu et al Blood. 2001 Dec 15;98(13):3778-83. doi:

    Insert

    BCR-FGFR1 (BCR Human)
    

    Expression

    Mammalian

    Available Since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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