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MECP2 - P152R in pENTR

Plasmid

#216595

Purpose

gateway cloning donor vector containing MECP2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

MECP2 (MECP2 Human)

Use

Gateway entry plasmid

Mutation

P152R

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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MECP2 - R91W in pENTR

Plasmid

#216593

Purpose

gateway cloning donor vector containing MECP2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

MECP2 (MECP2 Human)

Use

Gateway entry plasmid

Mutation

R91W

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-LASV-GPC-c3xFLAG

Plasmid

#215689

Purpose

Produces the Lassa virus glycoprotein with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Acciani et al J Virol. 2017 Aug 24;91(18):e00574-17. d

Insert

LASV GP-FLAG

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-TST-EGFP-GPIDAF

Plasmid

#213712

Purpose

For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag and V5-tag

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TST-EGFP-GPIBY55

Plasmid

#213711

Purpose

For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an SV40 promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag and V5-tag

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk2a

Plasmid

#208983

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk2a (kcnk2a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Sars Cov 2 MPRO R188S mutantSARS-CoV-2 Mpro protease (R188S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204785

Purpose

Bacterial expression of Sars Cov 2 MPRO R188S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

R188S

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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ZIKV NS2B-NS3 protease (H115A- catalytically inactive); aliases: Zika Virus NS2B-NS3 fusion protein expressed with N-terminal Tw

Plasmid

#204791

Purpose

Bacterial expression of a fusion of Zika NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

Zika NS2B-NS3 fusion

Tags

6 His

Expression

Bacterial

Available Since

Jan. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDY1935

Plasmid

#212315

Purpose

Express ApmFnuc protein with sumotag

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

Isvmimi

Tags

6xHis-thrombin site-TwinStrepTag-SUMO

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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WNV NS2B-NS3 protease (based on PDB 2IJO, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204799

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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