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mCh-CRY2-sspB2

Plasmid

#223691

Purpose

PhoBIT2 component; sspB2 (sspB mutant A56F) fused to mCh-CRY2PHR

Depositor

Yubin Zhou

Article

Lee et al Nat Commun. 2025 Jul 28;16(1):6940. doi:

Insert

CRY2-sspB2(A56F) (CRY2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMVf

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_CG6867_nostop

Plasmid

#240238

Purpose

Gateway entry clone with CG6867 without stop codon

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

CG6867 (CG6867 Fly)

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBS-GMR-eya(shRNA)_EcoRImut

Plasmid

#240222

Purpose

pBS-GMR-eya(shRNA) vector with one of two EcoRI sites mutated (T->A)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Type

Empty backbone

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_BIP-sfGFP-TurboID-KDEL

Plasmid

#240223

Purpose

Gateway entry clone with ER-localized TurboID tagged with superfolder GFP (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

BIP-sfGFP-TurboID-KDEL

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPMEL-Nluc-hbb

Plasmid

#239862

Purpose

Template for in vitro transcription of secreted nanoluciferase, with signal peptide from PMEL, flanked by the human haemoglobin beta 5' and 3' UTRs.

Depositor

Catherine Jopling

Article

Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3

Insert

Secreted nanoluciferase, with signal peptide from PMEL (gp100), flanked by human haemoglobin beta 5' and 3' UTRs

Use

Luciferase

Promoter

T7 (included as part of insert)

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV18

Plasmid

#237942

Purpose

Encodes TRBV18 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV18-TRAC (TRBV18 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_010_psbD_5'Hom_Cr

Plasmid

#235788

Purpose

5' Homology psbD in the M1 position used for genome integration at the psbD locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the psbD locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_005_rbcL_5'Hom_Cr

Plasmid

#235786

Purpose

5' Homology rbcL in the M1 position used for genome integration at the rbcL locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Homology region of the rbcL locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA376 - pBA904 Puro-T2A-GFP NTC guide (pRCA360 backbone)

Plasmid

#238167

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1124

Plasmid

#235725

Purpose

Expression of human UVRAG (1-464) fused to human ATG14L BATS (413-492) in mammalian cells

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

UVRAG(1-464) fused to ATG14L BATS domain (413-492)

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pJB180

Plasmid

#235737

Purpose

Expression of mCherry- human RAB1A Q70L in mammalian cells

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

RAB1A (RAB1A Human)

Tags

mCherry

Expression

Mammalian

Mutation

Q70L

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-229E-nsp7

Plasmid

#168956

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-nsp7 (HCoV229Egp1 )

Use

Gateway-compatible entry vector

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-229E-nsp1

Plasmid

#168952

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-nsp1 (HCoV229Egp1 )

Use

Gateway-compatible entry vector

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-NS2

Plasmid

#168942

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-NS2 (ns2 )

Use

Gateway-compatible entry vector

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1296

Plasmid

#235710

Purpose

Expression of mCherry-human RAB5A Q79L in mammalian cells

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

RAB5 (RAB5A Human)

Tags

mCherry

Expression

Mammalian

Mutation

Q79L, aa 2-215

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-nsp15

Plasmid

#168940

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-nsp15 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-nsp16

Plasmid

#168901

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-nsp16 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-nsp15

Plasmid

#168900

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-nsp15 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-2-nsp14

Plasmid

#168874

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-nsp14 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-2-nsp10

Plasmid

#168873

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-nsp10 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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