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Showing: 6761 - 6780 of 27313 results

  6. Level 1 P1 OsActinP:Hygint:NosT

    Plasmid
    #165423
    Purpose
    GoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassette
    Depositor
    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
    Article
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Insert
    Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
    Use
    Synthetic Biology; L1 p1(r) golden gate (moclo) h…
    Promoter
    Os Actin promoter :: Hygromycin resistance gene (…
    Available Since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165423-plasmid-map-sequence-id-331267

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  12. ERAP1

    Plasmid
    #39174
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ERAP1 (ERAP1 Human)
    Use
    Baculovirus expression
    Tags
    TEV-His10-Flag
    Promoter
    polyhedrin
    Available Since
    June 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA

    Plasmid
    #105000
    Purpose
    Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.
    Insert
    Renilla luciferase
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Promoter
    TetCMV
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105000-plasmid-map-sequence-id-203163

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  14. pACEC3GH (Apr)

    Plasmid
    #110085
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110085-plasmid-map-sequence-id-211188

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  17. pJAT7

    Plasmid
    #204292
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-DsRed
    Use
    CRISPR
    Promoter
    3XP3
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204292-plasmid-map-sequence-id-405844

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  18. pMAL-SH3(2)-5R

    Plasmid
    #112089
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.
    Depositor
    Michael Rosen
    Article
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    SH3(2)-5R (NCK1 Human)
    Tags
    His6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only repeats of the second SH3…
    Promoter
    Lac
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112089-plasmid-map-sequence-id-236082

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  20. pLenti PGK Puro GFP-LC3B G120A

    Plasmid
    #123242
    Purpose
    Expression vector with PGK promoter for low expression of EGFP-LC3B G120A. For lentivirus production and stable transduction in mammalian cells.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glycine 120 to Alanine
    Promoter
    PGK
    Available Since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123242-plasmid-map-sequence-id-244541

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Showing: 6761 - 6780 of 27313 results