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pCMV_PACLight1

Plasmid

#197864

Purpose

Mammalian expression of PACLight1 biosensor for PACAP (Pituitary Adenylate Cyclase Activating Peptide)

Depositor

Tommaso Patriarchi

Article

Cola et al Elife. 2024 Aug 15;13:RP96496. doi: 10.7

Insert

PACLight1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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AAV.hSyn.GLPLight1

Plasmid

#187466

Purpose

Expresses GLPLight1 in neurons

Depositor

Tommaso Patriarchi

Article

Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

Insert

GLPLight1

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

human Synapsin-1

Available since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pXylA-agrCA-I

Plasmid

#53437

Purpose

xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pT7-agrBD-I

Plasmid

#53439

Purpose

T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrBD locus

Use

Synthetic Biology

Tags

V5 epitope, 6x HIS (not expressed on agrB or agrD…

Expression

Bacterial

Mutation

none

Promoter

T7-lac

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pHFX-B60-EPSUD

Plasmid

#174390

Purpose

Plasmids for gene knockout were constructed based on suicide plasmid pHFX-B60

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

EPSUD

Use

Archaeal gene knockout vector for haloferax medit…

Tags

Expression

Mutation

Promoter

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pXylA-agrCA-IV

Plasmid

#53438

Purpose

xylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pUAP1_Ta_Yr7_CDS_no_STOP

Plasmid

#141093

Purpose

Level 0 Golden gate module with the coding sequence of Cadenza-Yr7 without its STOP codon

Depositor

Cristobal Uauy

Article

Marchal et al Genes (Basel). 2020 Nov 26;11(12). pii:

Insert

Ta_Yr7

Use

Unspecified; Level 0 module for golden gate cloni…

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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L1-Ck1-hs2-l-chlo

Plasmid

#163938

Purpose

For integration in the chloroplast rbcL_trnR region: HS2-L, genome position: 56139_57662, Genbank accession no. MH635409

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Marchantia polymorpha rbcL_trnR region homologous sequence

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PROM5_Mprnpo_chlo

Plasmid

#163951

Purpose

L0 part - promoter

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Marchantia polymorpha chloroplast DNA-dependent RNA polymerase operon promoter (rpo) (genome position: 5595-5739)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2+-wbp2nlY282F

Plasmid

#172468

Purpose

expresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlY282F

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Tyrosine at aa282 to Phenylalanine

Promoter

SP6

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlF127P

Plasmid

#174507

Purpose

expresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlF127P

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Phenylalanine at aa127 to Proline

Promoter

SP6

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlT45A

Plasmid

#172464

Purpose

expresses protein with one aa substitution to affect potential phosphorylation

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlT45A

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Threonine at aa45 to Alanine

Promoter

SP6

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlY55F

Plasmid

#172466

Purpose

expresses protein with one aa substitution to affect potential phosphorylation in ITSM domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlY55F

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Tyrosine at aa55 to Phenylalanine

Promoter

SP6

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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AF0837(1-127)

Plasmid

#19092

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota (DOM34 Archaeoglobus fulgidus)

Use

Tags

C tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
AF0837(1-241)

Plasmid

#19095

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota (DOM34 Archaeoglobus fulgidus)

Use

Tags

C tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pCRII_TOPO_hNEAT1

Plasmid

#61518

Purpose

human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

Depositor

Archa Fox

Article

Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

Insert

NEAT1 (NEAT1 Human)

Use

General cloning vector

Tags

Expression

Mutation

*see comment below

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-RdLight1

Plasmid

#125706

Purpose

Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Insert

RdLight1

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03

Plasmid

#71535

Purpose

Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi, Sumihare Noji

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGE010

Plasmid

#71536

Purpose

For CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGE011

Plasmid

#71537

Purpose

For CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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