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Plasmid#63757PurposeEPEC eae probe. 1.1 kb Sa1I/StuI fragment of E2348/69 (from gene bank cosmid II E1) cloned into Sa1I/SmaI site of pUC18.DepositorInserteae probe
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK793
Plasmid#8827DepositorInsertTEV protease, S219V mutant
UseTagsHis, MBP (with TEV), and polyarginineExpressionBacterialMutationS219V mutation (improves stability)PromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSicoR (Puro) p65 shRNA1
Plasmid#22507DepositorInsertp65 shRNA1 (Rela Mouse)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRK1043
Plasmid#8835DepositorInserttobacco etch virus protease, catalytic domain
UseTagsMBP and polyarginineExpressionBacterialMutationAutolysis-resistant S219V mutantPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pLVX-IRES-hygro-EGFP-Stx17
Plasmid#174652PurposeVector for overexpression of EGFP-tagged Stx17 with IRES for hygromycin selection.DepositorInsertEGFP-Stx17 (STX17 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterCMVAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB80-2xFKBP-L-mCherry-CAAX
Plasmid#193713PurposeRapalog-inducible coupling to plasma membrane (CAAX) via FKBPDepositorInsert2xFKBP-L-mCherry-CAAX
UseTags2xFKBP-mCherryExpressionMammalianMutationmCherry: Met1 DeletedPromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB80-iLID-mCherry-RAB5
Plasmid#174624PurposeOptogenetic coupling to early endosomes (RAB5) via iLIDDepositorInsertiLID-mCherry-RAB5 (RAB5A Synthetic, Human)
UseTagsiLID-mCherryExpressionMammalianMutationmCherry: Met1Del; RAB5: Met1DelPromoterChicken beta-actinAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSicoR (Puro) p65 shRNA2
Plasmid#22508DepositorInsertp65 shRNA2 (Rela Mouse)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJK357
Plasmid#71568PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-GCP2
Plasmid#178068PurposeInsect cell expression of GCP2DepositorInsertGCP2 (TUBGCP2 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACCS3-1
Plasmid#78559Purpose4.7 kb HindIII ETEC CS3 fimbrial operon from pCS100 cloned into pACYC184.DepositorInsertcs3 operon
UseTagsExpressionBacterialMutationPromotertetAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVD447
Plasmid#78569PurposeE. coli E2348/69 LEE region (Type 3 Secretion System) cloned into pBluescriptKS.DepositorInsertlee operon
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK511
Plasmid#71591PurposeProduces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag, His222>Gln mutant (TpCSH6-H222Q)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationchanges histidine-222 to glutaminePromoterT7 promoterAvailable sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Jonah-1
Plasmid#228899PurposeJonah-1 localizes to the outer cyst wall of Acanthamoeba tagged with eGFPDepositorInsertJonah-1
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwn promoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Luke-2
Plasmid#228900PurposeLuke-2 localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFPDepositorInsertCarbohydrate binding domain cbm49 protein
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwnAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Leo-A
Plasmid#228901PurposeLeo-A localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFPDepositorInsertLeo-A
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwnAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
PTet-F30-Pepper aptamer-UTR1-mTurq2
Plasmid#227645PurposepTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.DepositorInsertF30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-ZZ-TEV-MZT2-3C-mEGFP
Plasmid#178074PurposeInsect cell expression of ZZ-TEV-MZT2-3C-mEGFPDepositorInsertMZT2A (MZT2A Human)
UseTagsPreScission-mEGFP and ZZ-TEVExpressionInsectMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only