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Plasmid#194460PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-JAG1 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmeEF1A_R
Plasmid#147347PurposeInsect Expression of DmeIF1ADepositorInsertDmeIF1A (eIF-1A Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeEF1A_R
Plasmid#147349PurposeInsect Expression of DmeIF1ADepositorInsertDmeIF1A (eIF-1A Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-ArgSen
Plasmid#184691PurposeLentiviral transfer plasmid for expression of ArgSenDepositorInsertArgSen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-ArgSen
Plasmid#184700PurposeLentiviral transfer plasmid for expression of ArgSenDepositorInsertArgSen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-pHCountdown
Plasmid#182301Purposelentiviral plasmid encoding pH-Countdown and puromycin selectionDepositorInsertpH countdown
UseLentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α -NWM-Node 1
Plasmid#154253Purposeexpresses reconstructed New World monkey retroCHMP3 Node 1 in mammalian cellsDepositorInsertreconstructed Node 1 of New World monkey retroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α -NWM-Node 2
Plasmid#154254Purposeexpresses reconstructed New World monkey retroCHMP3 Node 2 in mammalian cellsDepositorInsertreconstructed Node 2 of New World monkey retroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α -NWM-Node 3
Plasmid#154255Purposeexpresses reconstructed New World monkey retroCHMP3 Node 3 in mammalian cellsDepositorInsertreconstructed Node 3 of New World monkey retroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_MAPK9-P2A-Hygro_Barcode
Plasmid#170238PurposeBarcoded lentiviral vector to express MAPK9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertMAPK9 (MAPK9 Human)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
MS2592.EF1a.SLC6A1.S295L.RFP.P2A.PURO.JL.p163
Plasmid#170174PurposeLentiviral Expression of EF1a.SLC6A1.S295L.RFPDepositorInsertSLC6A1 (SLC6A1 Human)
UseLentiviralTagsExpressionMutationS295LPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
MS2592.EF1a.SLC6A1.A288V.P2A.PURO.JL.p166
Plasmid#170171PurposeLentiviral Expression of EF1a.SLC6A1.A288VDepositorInsertSLC6A1 (SLC6A1 Human)
UseLentiviralTagsExpressionMutationA288VPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-HaloSFPQY527A
Plasmid#166949PurposeLentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the EF1a promoterDepositorInsertSFPQ-Y527A (SFPQ Human)
UseLentiviralTagsHaloExpressionMutationSFPQ-Y527APromotereF1aAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD
Plasmid#162811PurposeControl STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.DepositorInsertBsmBI_(C1A)-Intein_DD
UsePiggybacTagsExpressionMammalianMutationIntein(Cys1,Ala)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)
Plasmid#162822PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)DepositorInsertUb-(N-TAG)CoV2_ORF3b(CoV1h)
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF3b(CoV1h)(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI
Plasmid#162803PurposeControl STELLA destination plasmid for N-terminally labelled proteinsDepositorInsertUb(G75,76A)-BsmBI
UsePiggybacTagsExpressionMammalianMutationUbiquitin (G75,76A)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD
Plasmid#162805PurposeSTELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integrationDepositorInsertUb-BsmBI-HA_IRES_Blasticidin
UsePiggybacTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-EGFPKir7.1
Plasmid#107181PurposeLentiviral particle generationDepositorInsertKCNJ13 (KCNJ13 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterEF1AAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Ccdc167
Plasmid#120242PurposeExpresses V5-tagged Ccdc167 in lentiviral vectorDepositorInsertCcdc167 (Ccdc167 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Tmem128
Plasmid#120239PurposeExpresses V5-tagged Tmem128 in lentiviral vectorDepositorInsertTmem128 (Tmem128 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only