Addgene

We narrowed to 1,127 results for: fret

Showing: 661 - 680 of 1127 results

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pcDNA3.1(+)mGAT1YFP28

Plasmid

#41673

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Use

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; 28 C-terminal-…

Promoter

CMV

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pQ54-VC

Plasmid

#175060

Purpose

expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ54) in mammalian cells

Depositor

Arnold Boersma

Article

Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

Insert

polyQ54 (HTT Human)

Use

Tags

mVenus + mCherry

Expression

Mammalian

Mutation

mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

Promoter

CMV Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pQ25-VC

Plasmid

#175062

Purpose

expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ25) in mammalian cells

Depositor

Arnold Boersma

Article

Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

Insert

polyQ25 (HTT Human)

Use

Tags

mVenus + mCherry

Expression

Mammalian

Mutation

mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

Promoter

CMV Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pQ39-VC

Plasmid

#175061

Purpose

expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ39) in mammalian cells

Depositor

Arnold Boersma

Article

Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

Insert

polyQ39 (HTT Human)

Use

Tags

mVenus + mCherry

Expression

Mammalian

Mutation

mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

Promoter

CMV Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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cpVenus-Gγ2

Plasmid

#69626

Purpose

The heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

cp173Venus-Ggamma2 (GNG2 Synthetic)

Use

Tags

ciruclar permutated Venus (cp173V)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pVC-DNAJB6b

Plasmid

#175065

Purpose

expresses mVenus and mCherry tagged DNAJB6b in mammalian cells

Depositor

Arnold Boersma

Article

Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

Insert

DNAJB6b (DNAJB6 Human)

Use

Tags

V5 and mVenus + mCherry

Expression

Mammalian

Mutation

mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

Promoter

CMV Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Chicken Mermaid S188

Plasmid

#53617

Purpose

Genetically encoded voltage sensor based on mUKG-mKOk FRET pair

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Chicken Mermaid S188

Use

Tags

Expression

Mammalian

Mutation

Gg-VSP contains an R153Q mutation and an amino ac…

Promoter

CMV

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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SNAP-ACE2

Plasmid

#178592

Purpose

Expresses SNAP-fused ACE2 protein that can be fluorescently labelled using SNAP substrates.

Depositor

Ralf Jockers

Article

Cecon et al Cell Chem Biol. 2021 Jul 2. pii: S2451-9

Insert

angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Flag and SNAP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAuroraA GFP-AURKA-GFP

Plasmid

#157765

Purpose

Expression of AURKA fused to GFP at both ends in mammalian cells under AurkA promoter

Depositor

Marc Tramier

Article

Bertolin et al ACS Sens. 2019 Aug 23;4(8):2018-2027. do

Insert

AURKA (AURKA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

promoter CMV replaced by AurkA promoter

Promoter

AURKA minimal

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)

Plasmid

#205098

Purpose

Cre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Bjorefeldt et al bioRxiv. 2023 Dec 4:2023.06.26.545546. d

Insert

mNeonGreen, eKL9h, and Volvox channelrhodopsin-1

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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mRuby2-Calreticulin-N-16

Plasmid

#55892

Purpose

Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Calreticulin (CALR Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mRuby2-Mito-7

Plasmid

#55905

Purpose

Localization: Mitochondria, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Mito (COX8A Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1(+)mGAT1YFP3

Plasmid

#41667

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Use

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; 3 C-terminal-m…

Promoter

CMV

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1YFP8

Plasmid

#41669

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Use

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; 8 C-terminal-m…

Promoter

CMV

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
mRuby2-CENPB-N-22

Plasmid

#55893

Purpose

Localization: Nucleus/Centromeres, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

CENPB (CENPB Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mRuby2-Calnexin-N-14

Plasmid

#55891

Purpose

Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Calnexin (CANX Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcdna DSG-2 TS

Plasmid

#89465

Purpose

DSG-2 tension sensor, using TSmod inserted in human DSG-2

Depositor

Daniel Conway

Article

Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

Insert

desmoglein-2 (DSG2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Clover-TOMM20-N-10

Plasmid

#56307

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

TOMM20 (TOMM20 Human)

Use

Tags

Clover

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mRuby2-TOMM20-N-10

Plasmid

#55913

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

TOMM20 (TOMM20 Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mRuby2-Actin-C-18

Plasmid

#55889

Purpose

Localization: Actin, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Actin (ACTB Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits