We narrowed to 3,601 results for: human cdnas

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pcDNA-Flag-LmoCas6

Plasmid

#126485

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-LmoCas8b2

Plasmid

#126482

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas8b2

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-LmoCas7

Plasmid

#126483

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas7

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-LmoCas5

Plasmid

#126484

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas5

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-VprBP (C590)

Plasmid

#133589

Purpose

Expresses human VprBP C590 mutant in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

Vpr (HIV-1) binding protein (DCAF1 Human)

Tags

HA

Expression

Mammalian

Mutation

C590 mutation of human VprBP lacking the N-termin…

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ULK2-K39I

Plasmid

#97076

Purpose

Expresses mutant ULK2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

Insert

ULK2 (ULK2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Lysine 39 to Isoleucine

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-VprBP (RARA)

Plasmid

#133587

Purpose

Expresses human VprBP RARA mutant in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

Vpr (HIV-1) binding protein (DCAF1 Human)

Tags

HA

Expression

Mammalian

Mutation

RARA mutation of human VprBP defective in DDB1 bi…

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas6

Plasmid

#106274

Purpose

Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasE

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7ADelta2bp-mGFP

Plasmid

#62636

Purpose

mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mEGFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Tags

mEGFP inserted in M3-M4 loop

Expression

Mammalian

Mutation

there is a 2-bp deletion in exon 6 of CHRFAM7A ca…

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CHRFAM7ADelta2bp-mCherry

Plasmid

#62638

Purpose

mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mCherry fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Expression

Mammalian

Mutation

there is a 2-bp deletion in exon 6 of CHRFAM7A an…

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only
cacna2d1 HA D491A pcDNA3

Plasmid

#206070

Purpose

expression of rat calcium channel alpha2delta-1 with a single HA tag inserted after aa549 and a D491A mutation that prevents Gabapentin binding

Depositor

Annette Dolphin

Article

Gumerov et al Proc Natl Acad Sci U S A. 2022 Mar 8;119

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Mutation

D491A

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-VprBP (N909)

Plasmid

#133588

Purpose

Expresses human VprBP N909 mutant in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

Vpr (HIV-1) binding protein (DCAF1 Human)

Tags

HA

Expression

Mammalian

Mutation

C590 mutation of human VprBP lacking the DDB1-bin…

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7ADelta2bp-YFP

Plasmid

#62637

Purpose

mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with YFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Expression

Mammalian

Mutation

there is a 2-bp deletion in exon 6 of CHRFAM7A an…

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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pY116 (pcDNA3.1-huMb2Cpf1)

Plasmid

#92292

Purpose

Expression of humanized Mb2Cpf1

Depositor

Feng Zhang

Insert

huMb2Cpf1

Tags

NLS and NLS-3xHA

Expression

Mammalian

Available Since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pY111 (pcDNA3.1-huTsCpf1)

Plasmid

#92267

Purpose

Expression of humanized TsCpf1

Depositor

Feng Zhang

Insert

huTsCpf1

Tags

NLS and NLS-3xHA

Expression

Mammalian

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pY117 (pcDNA3.1-huMb3Cpf1)

Plasmid

#92293

Purpose

Expression of humanized Mb3Cpf1

Depositor

Feng Zhang

Insert

huMb3Cpf1

Tags

NLS and NLS-3xHA

Expression

Mammalian

Available Since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pY113 (pcDNA3.1-huPb2Cpf1)

Plasmid

#92272

Purpose

Expression of humanized Pb2Cpf1

Depositor

Feng Zhang

Insert

huPb2Cpf1

Tags

NLS and NLS-3xHA

Expression

Mammalian

Available Since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pY119 (pcDNA3.1-huLb5Cpf1)

Plasmid

#92295

Purpose

Expression of humanized Lb5Cpf1

Depositor

Feng Zhang

Insert

huLb5Cpf1

Tags

NLS and NLS-3xHA

Expression

Mammalian

Available Since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pY118 (pcDNA3.1-huLb4Cpf1)

Plasmid

#92294

Purpose

Expression of humanized Lb4Cpf1

Depositor

Feng Zhang

Insert

huLb4Cpf1

Tags

NLS and NLS-3xHA

Expression

Mammalian

Available Since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pY124 (pcDNA3.1-huBsCpf1)

Plasmid

#92300

Purpose

Expression of humanized BsCpf1

Depositor

Feng Zhang

Insert

huBsCpf1

Tags

NLS and NLS-3xHA

Expression

Mammalian

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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