We narrowed to 2,731 results for: nags

1. pCX:B3-α4ΔICD

   Plasmid

   #208513

   Purpose

   ddGFP-B3-inserted Pcdhα4 lacking an intracellular domain (ICD)

   Depositor

   Takeharu Nagai

   Article

   Kanadome et al iScience. 2023 Jul 17;26(7):107238. doi:

   Insert

   Pcdha4 (Pcdha4 Mouse)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken β-actin

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCX:B1-α4ΔICD

   Plasmid

   #208511

   Purpose

   ddGFP-B1-inserted Pcdhα4 lacking an intracellular domain (ICD)

   Depositor

   Takeharu Nagai

   Article

   Kanadome et al iScience. 2023 Jul 17;26(7):107238. doi:

   Insert

   Pcdha4 (Pcdha4 Mouse)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken β-actin

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCX:α4ΔICD-EC4-GA

   Plasmid

   #208512

   Purpose

   ddGFP-A-inserted Pcdhα4 lacking an intracellular domain (ICD)

   Depositor

   Takeharu Nagai

   Article

   Kanadome et al iScience. 2023 Jul 17;26(7):107238. doi:

   Insert

   Pcdha4 (Pcdha4 Mouse)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken β-actin

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEU3-NII-HxHLICNot

   Plasmid

   #140183

   Purpose

   Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 12xHis-tag and NotI restriction site

   Depositor

   Tamás Mészáros

   Article

   Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

   Type

   Empty backbone

   Use

   Expression vector for a cell-free system

   Tags

   12xHis

   Expression

   Mutation

   Promoter

   T7

   Available since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CeNL(Ca2+)_110μ-pcDNA3

   Plasmid

   #111929

   Purpose

   Cyan color bright luminescent calcium indicator with low affinity

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_110μ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E31D, F92W, E104D. D133E at CaM

   Promoter

   CMV

   Available since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET15b+R2(L171C)

   Plasmid

   #108239

   Purpose

   Expresses PdR-PCNA2(L171C) fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PdR-PCNA2(L171C) fusion protein
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   PCNA2,L171C

   Promoter

   T7 promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET15T+1X

   Plasmid

   #108188

   Purpose

   PCNA1-PdX fusion protein

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PCNA1-PdX fusion protein
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   C73S/C85S (PdX)

   Promoter

   T7 promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMAL-SsoPCNA3

   Plasmid

   #107069

   Purpose

   Expresses S. solfataricus PCNA3 fused to MBP in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

   Insert

   S. solfataricus PCNA3
   

   Use

   Tags

   6xHis-thrombin cleavage site and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPD1R

   Plasmid

   #86969

   Purpose

   Expresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

   Insert

   PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E175A/A176R in PTDH

   Promoter

   T7 promoter

   Available since

   March 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p1G108CP20X

    Plasmid

    #85096

    Purpose

    Expresses PCNA1 variant (G108C) fused to putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    The G108C variant of PCNA1 fused to putidaredoxin
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    G108C in PCNA1

    Promoter

    T7 promoter

    Available since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p3R112C/T180C-GG

    Plasmid

    #85093

    Purpose

    Host vector to fuse a gene at the C-terminus of PCNA3 variant (R112C/T180C)

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Arginine 112 to Cysteine and Threonine 18…

    Promoter

    T7 promoter

    Available since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p1G108C-GG

    Plasmid

    #85092

    Purpose

    Host vector to fuse a gene at the C-terminus of PCNA1 variant (G108C)

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    DNA polymerase sliding clamp 1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Glycine 108 to Cysteine

    Promoter

    T7 promoter

    Available since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET11+PCNA3-CYP119

    Plasmid

    #66136

    Purpose

    Expresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET11+PCNA3-CYP119(T214V)

    Plasmid

    #66137

    Purpose

    Expresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    T214V

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET15b+PCNA3 T180C

    Plasmid

    #66181

    Purpose

    Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    T180C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET15b+PCNA3 R112C

    Plasmid

    #66180

    Purpose

    Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET15b+PCNA2 E105C

    Plasmid

    #66176

    Purpose

    Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 2
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E105C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET11+PCNA3-CYP119(D77R/T214V)

    Plasmid

    #66138

    Purpose

    Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    D77R, T214V

    Promoter

    T7 promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pASKt15C+SrtA5woH

    Plasmid

    #65019

    Purpose

    Expresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, D160N, D165A, K19…

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTH1-SaSrtA-KQ

    Plasmid

    #64982

    Purpose

    Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K, E108Q

    Promoter

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge